Actions of phenylglycine derivatives at L-AP4 receptors in retinal ON bipolar cells

Wallace B Thoreson, T. J. Velte, R. F. Miller

Research output: Contribution to journalArticle

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Abstract

Phenylglycine derivatives can act as agonists or antagonists at different metabotropic glutamate receptor (mGluR) subtypes, including subtypes sensitive to l-2-amino-4-phosphonobutyric acid (L-AP4). We examined the pharmacology of four phenylglycines at L-AP4 receptors in ON bipolar cells of the amphibian retina in situ. As previously shown for S-4-carboxy-3-hydroxyphenylglycine (S-4C3H-PG) (Thoreson W. B. and Miller R. F., J. Gen. Physiol. 103, 1019-1034, 1994), whole cell recordings indicate that S-3-carboxy-4hydroxyphenylglycine (S-3C4H-PG) and S-4-carboxyphenylglycine (S-4C-PG) are L-AP4 receptor agonists in retina. Concentration-response curves for these compounds were obtained using the b-wave of the electroretinogram (ERG) as an assay for ON bipolar cell activity. The rank-order potency and IC50 values obtained were: S-4C-PG (204 μM) > S-4C3H-PG (399 μM) ≥ S-3C4H-PG (558 μM). At 1 mM, RS-α-methyl4-carboxyphenylglycine (RS-M4C-PG) suppressed the b-wave by less than 20%. This weak effect is attributed to agonist actions of RS-M4C-PG. The agonist actions of phenylglycines in retina are different from their effects at L-AP4 receptors in spinal cord or the expressed L-AP4-sensitive receptor subtype, mGluR4 (Kemp et al, Eur. J. Pharmac. Molec. Pharmac., 266, 187-192, 1994; Thomsen et al., Eur. J. Pharmac. Molec. Pharmac., 267, 77-84, 1994; Hayashi et al., J. Neurosci., 14, 3370-3377, 1994). The results are therefore consistent with the proposal that there are multiple L-AP4-sensitive mGluR sybtypes and indicate that phenylglycine derivatives may be useful for pharmacologically discriminating among these sybtypes.

Original languageEnglish (US)
Pages (from-to)27-34
Number of pages8
JournalNeuropharmacology
Volume34
Issue number1
DOIs
StatePublished - Jan 1 1995

Fingerprint

Retinal Bipolar Cells
Retina
Metabotropic Glutamate Receptors
Amphibians
Patch-Clamp Techniques
Inhibitory Concentration 50
2-amino-4-phosphonobutanoic acid receptor
2-amino-4-phosphono-propinate
Spinal Cord
Pharmacology

Keywords

  • APB receptor
  • Metabotropic glutamate receptor
  • amphibian
  • electroretinogram
  • phenylglycines
  • retina
  • whole cell patch clamp recording

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Pharmacology
  • Drug Discovery

Cite this

Actions of phenylglycine derivatives at L-AP4 receptors in retinal ON bipolar cells. / Thoreson, Wallace B; Velte, T. J.; Miller, R. F.

In: Neuropharmacology, Vol. 34, No. 1, 01.01.1995, p. 27-34.

Research output: Contribution to journalArticle

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abstract = "Phenylglycine derivatives can act as agonists or antagonists at different metabotropic glutamate receptor (mGluR) subtypes, including subtypes sensitive to l-2-amino-4-phosphonobutyric acid (L-AP4). We examined the pharmacology of four phenylglycines at L-AP4 receptors in ON bipolar cells of the amphibian retina in situ. As previously shown for S-4-carboxy-3-hydroxyphenylglycine (S-4C3H-PG) (Thoreson W. B. and Miller R. F., J. Gen. Physiol. 103, 1019-1034, 1994), whole cell recordings indicate that S-3-carboxy-4hydroxyphenylglycine (S-3C4H-PG) and S-4-carboxyphenylglycine (S-4C-PG) are L-AP4 receptor agonists in retina. Concentration-response curves for these compounds were obtained using the b-wave of the electroretinogram (ERG) as an assay for ON bipolar cell activity. The rank-order potency and IC50 values obtained were: S-4C-PG (204 μM) > S-4C3H-PG (399 μM) ≥ S-3C4H-PG (558 μM). At 1 mM, RS-α-methyl4-carboxyphenylglycine (RS-M4C-PG) suppressed the b-wave by less than 20{\%}. This weak effect is attributed to agonist actions of RS-M4C-PG. The agonist actions of phenylglycines in retina are different from their effects at L-AP4 receptors in spinal cord or the expressed L-AP4-sensitive receptor subtype, mGluR4 (Kemp et al, Eur. J. Pharmac. Molec. Pharmac., 266, 187-192, 1994; Thomsen et al., Eur. J. Pharmac. Molec. Pharmac., 267, 77-84, 1994; Hayashi et al., J. Neurosci., 14, 3370-3377, 1994). The results are therefore consistent with the proposal that there are multiple L-AP4-sensitive mGluR sybtypes and indicate that phenylglycine derivatives may be useful for pharmacologically discriminating among these sybtypes.",
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AB - Phenylglycine derivatives can act as agonists or antagonists at different metabotropic glutamate receptor (mGluR) subtypes, including subtypes sensitive to l-2-amino-4-phosphonobutyric acid (L-AP4). We examined the pharmacology of four phenylglycines at L-AP4 receptors in ON bipolar cells of the amphibian retina in situ. As previously shown for S-4-carboxy-3-hydroxyphenylglycine (S-4C3H-PG) (Thoreson W. B. and Miller R. F., J. Gen. Physiol. 103, 1019-1034, 1994), whole cell recordings indicate that S-3-carboxy-4hydroxyphenylglycine (S-3C4H-PG) and S-4-carboxyphenylglycine (S-4C-PG) are L-AP4 receptor agonists in retina. Concentration-response curves for these compounds were obtained using the b-wave of the electroretinogram (ERG) as an assay for ON bipolar cell activity. The rank-order potency and IC50 values obtained were: S-4C-PG (204 μM) > S-4C3H-PG (399 μM) ≥ S-3C4H-PG (558 μM). At 1 mM, RS-α-methyl4-carboxyphenylglycine (RS-M4C-PG) suppressed the b-wave by less than 20%. This weak effect is attributed to agonist actions of RS-M4C-PG. The agonist actions of phenylglycines in retina are different from their effects at L-AP4 receptors in spinal cord or the expressed L-AP4-sensitive receptor subtype, mGluR4 (Kemp et al, Eur. J. Pharmac. Molec. Pharmac., 266, 187-192, 1994; Thomsen et al., Eur. J. Pharmac. Molec. Pharmac., 267, 77-84, 1994; Hayashi et al., J. Neurosci., 14, 3370-3377, 1994). The results are therefore consistent with the proposal that there are multiple L-AP4-sensitive mGluR sybtypes and indicate that phenylglycine derivatives may be useful for pharmacologically discriminating among these sybtypes.

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