Acrosome reaction is subfamily specific in sea star fertilization

Mia Nakachi, Hideaki Moriyama, Motonori Hoshi, Midori Matsumoto

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

In the fertilization process of sea stars, sperm is activated to go through the acrosome reaction before cell fusion. We focused on induction of the acrosome reaction as a key process in fertilization. Six species of sea stars were used in this study: Asterias amurensis, Asterias rubens, Asterias forbesi, Aphelasterias japonica, Distolasterias nipon, and Asterina pectinifera. Acrosome reaction assays indicate that the acrosome reaction can be induced across species within Asteriinae subfamily. However, cross-fertilization assays indicate that sea stars have species specificity in fertilization. Therefore, steps after the acrosome reaction are responsible for the species specificity. To explain acrosome reaction subfamily specificity at the molecular level, the sugar components of egg jelly were examined and analyzed by principal component analysis. A. amurensis and A. forbesi belong to the same induction group of the acrosome reaction. D. nipon and An. pectinifera are in a unique group. Enzyme-linked immunosorbent assays indicate that Asteriinae subfamily share a common glycan structure, the Fragment 1 of Acrosome Reaction-Inducing Substance from A. amurensis. Fragment 1 plays an important role in the subfamily specificity of acrosome reaction induction. In addition, A. amurensis sperm activating peptide was recognized by sperm from the same superorder. These results demonstrate that the specificity of acrosome reaction induction is present at the subfamily level in sea stars.

Original languageEnglish (US)
Pages (from-to)597-604
Number of pages8
JournalDevelopmental Biology
Volume298
Issue number2
DOIs
StatePublished - Oct 15 2006

Fingerprint

Starfish
Acrosome Reaction
Fertilization
Asterias
Spermatozoa
Species Specificity
Asterina
Cell Fusion
Principal Component Analysis
Polysaccharides
Ovum
Enzyme-Linked Immunosorbent Assay

Keywords

  • Acrosome reaction
  • Aphelasterias japonica
  • Asterias amurensis
  • Asterias forbesi
  • Asterias rubens
  • Asterina pectinifera
  • Distolasterias nipon
  • Egg jelly
  • Fertilization
  • Species specificity

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Cite this

Acrosome reaction is subfamily specific in sea star fertilization. / Nakachi, Mia; Moriyama, Hideaki; Hoshi, Motonori; Matsumoto, Midori.

In: Developmental Biology, Vol. 298, No. 2, 15.10.2006, p. 597-604.

Research output: Contribution to journalArticle

Nakachi, Mia ; Moriyama, Hideaki ; Hoshi, Motonori ; Matsumoto, Midori. / Acrosome reaction is subfamily specific in sea star fertilization. In: Developmental Biology. 2006 ; Vol. 298, No. 2. pp. 597-604.
@article{5a72ec858f144b8087bb4c3d00c68696,
title = "Acrosome reaction is subfamily specific in sea star fertilization",
abstract = "In the fertilization process of sea stars, sperm is activated to go through the acrosome reaction before cell fusion. We focused on induction of the acrosome reaction as a key process in fertilization. Six species of sea stars were used in this study: Asterias amurensis, Asterias rubens, Asterias forbesi, Aphelasterias japonica, Distolasterias nipon, and Asterina pectinifera. Acrosome reaction assays indicate that the acrosome reaction can be induced across species within Asteriinae subfamily. However, cross-fertilization assays indicate that sea stars have species specificity in fertilization. Therefore, steps after the acrosome reaction are responsible for the species specificity. To explain acrosome reaction subfamily specificity at the molecular level, the sugar components of egg jelly were examined and analyzed by principal component analysis. A. amurensis and A. forbesi belong to the same induction group of the acrosome reaction. D. nipon and An. pectinifera are in a unique group. Enzyme-linked immunosorbent assays indicate that Asteriinae subfamily share a common glycan structure, the Fragment 1 of Acrosome Reaction-Inducing Substance from A. amurensis. Fragment 1 plays an important role in the subfamily specificity of acrosome reaction induction. In addition, A. amurensis sperm activating peptide was recognized by sperm from the same superorder. These results demonstrate that the specificity of acrosome reaction induction is present at the subfamily level in sea stars.",
keywords = "Acrosome reaction, Aphelasterias japonica, Asterias amurensis, Asterias forbesi, Asterias rubens, Asterina pectinifera, Distolasterias nipon, Egg jelly, Fertilization, Species specificity",
author = "Mia Nakachi and Hideaki Moriyama and Motonori Hoshi and Midori Matsumoto",
year = "2006",
month = "10",
day = "15",
doi = "10.1016/j.ydbio.2006.07.009",
language = "English (US)",
volume = "298",
pages = "597--604",
journal = "Developmental Biology",
issn = "0012-1606",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Acrosome reaction is subfamily specific in sea star fertilization

AU - Nakachi, Mia

AU - Moriyama, Hideaki

AU - Hoshi, Motonori

AU - Matsumoto, Midori

PY - 2006/10/15

Y1 - 2006/10/15

N2 - In the fertilization process of sea stars, sperm is activated to go through the acrosome reaction before cell fusion. We focused on induction of the acrosome reaction as a key process in fertilization. Six species of sea stars were used in this study: Asterias amurensis, Asterias rubens, Asterias forbesi, Aphelasterias japonica, Distolasterias nipon, and Asterina pectinifera. Acrosome reaction assays indicate that the acrosome reaction can be induced across species within Asteriinae subfamily. However, cross-fertilization assays indicate that sea stars have species specificity in fertilization. Therefore, steps after the acrosome reaction are responsible for the species specificity. To explain acrosome reaction subfamily specificity at the molecular level, the sugar components of egg jelly were examined and analyzed by principal component analysis. A. amurensis and A. forbesi belong to the same induction group of the acrosome reaction. D. nipon and An. pectinifera are in a unique group. Enzyme-linked immunosorbent assays indicate that Asteriinae subfamily share a common glycan structure, the Fragment 1 of Acrosome Reaction-Inducing Substance from A. amurensis. Fragment 1 plays an important role in the subfamily specificity of acrosome reaction induction. In addition, A. amurensis sperm activating peptide was recognized by sperm from the same superorder. These results demonstrate that the specificity of acrosome reaction induction is present at the subfamily level in sea stars.

AB - In the fertilization process of sea stars, sperm is activated to go through the acrosome reaction before cell fusion. We focused on induction of the acrosome reaction as a key process in fertilization. Six species of sea stars were used in this study: Asterias amurensis, Asterias rubens, Asterias forbesi, Aphelasterias japonica, Distolasterias nipon, and Asterina pectinifera. Acrosome reaction assays indicate that the acrosome reaction can be induced across species within Asteriinae subfamily. However, cross-fertilization assays indicate that sea stars have species specificity in fertilization. Therefore, steps after the acrosome reaction are responsible for the species specificity. To explain acrosome reaction subfamily specificity at the molecular level, the sugar components of egg jelly were examined and analyzed by principal component analysis. A. amurensis and A. forbesi belong to the same induction group of the acrosome reaction. D. nipon and An. pectinifera are in a unique group. Enzyme-linked immunosorbent assays indicate that Asteriinae subfamily share a common glycan structure, the Fragment 1 of Acrosome Reaction-Inducing Substance from A. amurensis. Fragment 1 plays an important role in the subfamily specificity of acrosome reaction induction. In addition, A. amurensis sperm activating peptide was recognized by sperm from the same superorder. These results demonstrate that the specificity of acrosome reaction induction is present at the subfamily level in sea stars.

KW - Acrosome reaction

KW - Aphelasterias japonica

KW - Asterias amurensis

KW - Asterias forbesi

KW - Asterias rubens

KW - Asterina pectinifera

KW - Distolasterias nipon

KW - Egg jelly

KW - Fertilization

KW - Species specificity

UR - http://www.scopus.com/inward/record.url?scp=33749155784&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33749155784&partnerID=8YFLogxK

U2 - 10.1016/j.ydbio.2006.07.009

DO - 10.1016/j.ydbio.2006.07.009

M3 - Article

VL - 298

SP - 597

EP - 604

JO - Developmental Biology

JF - Developmental Biology

SN - 0012-1606

IS - 2

ER -