Acquisition of CD80 (B7-1) by T cells

H. Sabzevari, J. Kantor, A. Jaigirdar, Y. Tagaya, M. Naramura, J. W. Hodge, J. Bernon, J. Schlom

Research output: Contribution to journalArticle

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Abstract

Activation of T cells usually requires two signals. Signal 1 is mediated via a peptide-MHC on the APC; signal 2 is mediated via a costimulatory molecule on the APC surface. We demonstrate here that naive CD4+ T cells actually acquire the costimulatory molecule CD80 (B7-1) from syngeneic APCs after activation. This phenomenon was demonstrated showing acquisition of CD80 by T cells from CD80/CD86 (B7-2) knockout mice, and by treating T cells with cyclohexamide to further rule out endogenous expression of CD80 by T cells. Moreover, no CD80 mRNA could be detected in T cells that had acquired CD80. The amount of acquisition of CD80 by T cells was shown to be directly related to both the strength of signal 1 and the amount of CD80 on the APC. Specificity of this acquisition was also shown by the lack of acquisition by T cells from CD28 knockout mice (implicating CD28 in this process), the lack of acquisition of CD40 (another molecule on the APC surface) by T cells, and confocal microscopy studies. We demonstrate for the first time that 1) naive T cells, following acquisition of CD80 from APCs, were themselves shown to be capable of acting as APCs; and 2) memory T cells that have acquired CD80 from APCs undergo apoptosis in the presence of increased levels of signal 1. Thus we demonstrate both immunostimulatory and immunoregulatory functions as a result of CD80 acquisition by different T cell populations.

Original languageEnglish (US)
Pages (from-to)2505-2513
Number of pages9
JournalJournal of Immunology
Volume166
Issue number4
DOIs
StatePublished - Feb 15 2001

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T-Lymphocytes
Knockout Mice
CD80 Antigens
Confocal Microscopy
Apoptosis
Messenger RNA
Peptides
Population

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Sabzevari, H., Kantor, J., Jaigirdar, A., Tagaya, Y., Naramura, M., Hodge, J. W., ... Schlom, J. (2001). Acquisition of CD80 (B7-1) by T cells. Journal of Immunology, 166(4), 2505-2513. https://doi.org/10.4049/jimmunol.166.4.2505

Acquisition of CD80 (B7-1) by T cells. / Sabzevari, H.; Kantor, J.; Jaigirdar, A.; Tagaya, Y.; Naramura, M.; Hodge, J. W.; Bernon, J.; Schlom, J.

In: Journal of Immunology, Vol. 166, No. 4, 15.02.2001, p. 2505-2513.

Research output: Contribution to journalArticle

Sabzevari, H, Kantor, J, Jaigirdar, A, Tagaya, Y, Naramura, M, Hodge, JW, Bernon, J & Schlom, J 2001, 'Acquisition of CD80 (B7-1) by T cells', Journal of Immunology, vol. 166, no. 4, pp. 2505-2513. https://doi.org/10.4049/jimmunol.166.4.2505
Sabzevari H, Kantor J, Jaigirdar A, Tagaya Y, Naramura M, Hodge JW et al. Acquisition of CD80 (B7-1) by T cells. Journal of Immunology. 2001 Feb 15;166(4):2505-2513. https://doi.org/10.4049/jimmunol.166.4.2505
Sabzevari, H. ; Kantor, J. ; Jaigirdar, A. ; Tagaya, Y. ; Naramura, M. ; Hodge, J. W. ; Bernon, J. ; Schlom, J. / Acquisition of CD80 (B7-1) by T cells. In: Journal of Immunology. 2001 ; Vol. 166, No. 4. pp. 2505-2513.
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