Acid hydrolysis and fermentation of microalgal starches to ethanol by the yeast Saccharomyces cerevisiae

Matthew J. Scholz, Mark R. Riley, Joel L. Cuello

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Many studies on the use of microalgal biomass to generate lipid and starch for production of transportation fuels assume ready conversion of starch to ethanol. However, few have shown data on this process. Here, biomass from the wall-deficient cells of Chlamydomonas reinhardtii mutant cw15 was sequentially treated with 70 °C ethanol and 60 °C hexane prior to sulfuric acid hydrolysis at 121 °C and 2 atm. Hydrolysis was monitored over time until no further starch breakdown was observed. An estimated 30% of the dry algal biomass was hydrolyzed for fermentation. This hydrolyzed starch was successfully fermented into ethanol by Saccharomyces cerevisiae to a maximum concentration of 0.87 ± 0.01% (m/v) at a maximum rate of 14 ± 1 mL/g h in 28 h. The observed yield coefficient of ethanol from glucose was 0.44 (g/g). Ethanol and what appeared to be glycerol were produced in equal proportions.

Original languageEnglish (US)
Pages (from-to)59-65
Number of pages7
JournalBiomass and Bioenergy
Volume48
DOIs
StatePublished - Jan 1 2013

Fingerprint

acid hydrolysis
Starch
starch
Yeast
Fermentation
fermentation
yeast
ethanol
hydrolysis
Saccharomyces cerevisiae
Hydrolysis
Ethanol
yeasts
Acids
acid
Biomass
biomass
Chlamydomonas reinhardtii
sulfuric acid
Hexane

Keywords

  • Chlamydomonas reinhardtii
  • Cw15
  • Ethanol
  • Fermentation
  • Hydrolysis
  • Saccharomyces cerevisiae

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Forestry
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

Cite this

Acid hydrolysis and fermentation of microalgal starches to ethanol by the yeast Saccharomyces cerevisiae. / Scholz, Matthew J.; Riley, Mark R.; Cuello, Joel L.

In: Biomass and Bioenergy, Vol. 48, 01.01.2013, p. 59-65.

Research output: Contribution to journalArticle

@article{7745cbd5786049759ae1b435dd992636,
title = "Acid hydrolysis and fermentation of microalgal starches to ethanol by the yeast Saccharomyces cerevisiae",
abstract = "Many studies on the use of microalgal biomass to generate lipid and starch for production of transportation fuels assume ready conversion of starch to ethanol. However, few have shown data on this process. Here, biomass from the wall-deficient cells of Chlamydomonas reinhardtii mutant cw15 was sequentially treated with 70 °C ethanol and 60 °C hexane prior to sulfuric acid hydrolysis at 121 °C and 2 atm. Hydrolysis was monitored over time until no further starch breakdown was observed. An estimated 30{\%} of the dry algal biomass was hydrolyzed for fermentation. This hydrolyzed starch was successfully fermented into ethanol by Saccharomyces cerevisiae to a maximum concentration of 0.87 ± 0.01{\%} (m/v) at a maximum rate of 14 ± 1 mL/g h in 28 h. The observed yield coefficient of ethanol from glucose was 0.44 (g/g). Ethanol and what appeared to be glycerol were produced in equal proportions.",
keywords = "Chlamydomonas reinhardtii, Cw15, Ethanol, Fermentation, Hydrolysis, Saccharomyces cerevisiae",
author = "Scholz, {Matthew J.} and Riley, {Mark R.} and Cuello, {Joel L.}",
year = "2013",
month = "1",
day = "1",
doi = "10.1016/j.biombioe.2012.10.026",
language = "English (US)",
volume = "48",
pages = "59--65",
journal = "Biomass and Bioenergy",
issn = "0961-9534",
publisher = "Elsevier Limited",

}

TY - JOUR

T1 - Acid hydrolysis and fermentation of microalgal starches to ethanol by the yeast Saccharomyces cerevisiae

AU - Scholz, Matthew J.

AU - Riley, Mark R.

AU - Cuello, Joel L.

PY - 2013/1/1

Y1 - 2013/1/1

N2 - Many studies on the use of microalgal biomass to generate lipid and starch for production of transportation fuels assume ready conversion of starch to ethanol. However, few have shown data on this process. Here, biomass from the wall-deficient cells of Chlamydomonas reinhardtii mutant cw15 was sequentially treated with 70 °C ethanol and 60 °C hexane prior to sulfuric acid hydrolysis at 121 °C and 2 atm. Hydrolysis was monitored over time until no further starch breakdown was observed. An estimated 30% of the dry algal biomass was hydrolyzed for fermentation. This hydrolyzed starch was successfully fermented into ethanol by Saccharomyces cerevisiae to a maximum concentration of 0.87 ± 0.01% (m/v) at a maximum rate of 14 ± 1 mL/g h in 28 h. The observed yield coefficient of ethanol from glucose was 0.44 (g/g). Ethanol and what appeared to be glycerol were produced in equal proportions.

AB - Many studies on the use of microalgal biomass to generate lipid and starch for production of transportation fuels assume ready conversion of starch to ethanol. However, few have shown data on this process. Here, biomass from the wall-deficient cells of Chlamydomonas reinhardtii mutant cw15 was sequentially treated with 70 °C ethanol and 60 °C hexane prior to sulfuric acid hydrolysis at 121 °C and 2 atm. Hydrolysis was monitored over time until no further starch breakdown was observed. An estimated 30% of the dry algal biomass was hydrolyzed for fermentation. This hydrolyzed starch was successfully fermented into ethanol by Saccharomyces cerevisiae to a maximum concentration of 0.87 ± 0.01% (m/v) at a maximum rate of 14 ± 1 mL/g h in 28 h. The observed yield coefficient of ethanol from glucose was 0.44 (g/g). Ethanol and what appeared to be glycerol were produced in equal proportions.

KW - Chlamydomonas reinhardtii

KW - Cw15

KW - Ethanol

KW - Fermentation

KW - Hydrolysis

KW - Saccharomyces cerevisiae

UR - http://www.scopus.com/inward/record.url?scp=84871576986&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84871576986&partnerID=8YFLogxK

U2 - 10.1016/j.biombioe.2012.10.026

DO - 10.1016/j.biombioe.2012.10.026

M3 - Article

AN - SCOPUS:84871576986

VL - 48

SP - 59

EP - 65

JO - Biomass and Bioenergy

JF - Biomass and Bioenergy

SN - 0961-9534

ER -