Acclimation to Cu in fathead minnows

Does age influence the response?

Marlo K. Sellin, Erik Tate-Boldt, Alan S Kolok

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

This study had two primary objectives. The first was to determine if the length of exposure necessary for acclimation to Cu to develop in larval fathead minnows (Pimephales promelas) is different than that for juveniles. The second objective was to determine whether the acclimatory response, as determined by organism survival, is consistent with acclimation as determined by whole-body Na+. Six experiments were conducted: four using larval (<20-d-old) and two using juvenile (<60-d-old) fathead minnows. Within each experiment, fish were allocated to one of four groups: unexposed, continuously exposed, episodically exposed or naïvely exposed. The continuous group was exposed to a sublethal Cu exposure (125 μg/L) for 8, 12, 16 or 20 d and then subjected to a survival test at a lethal dose (375 μg/L). Fish in the episodic group were exposed to the sublethal dose for either 4 or 8 d, given a depuration period of varying lengths (4-16 d) then subjected to a survival test. Naïve minnows were maintained in clean water then given the survival challenge. Results from survival tests show that the larvae acclimate after only a 4-d sublethal exposure to Cu. In contrast, juveniles require a 16-d exposure to acclimate. Once acclimation had developed, there was a strong relationship between larval survival and whole-body Na+. Acclimated larvae maintained whole-body Na+ relative to unexposed fish, while unacclimated larvae did not. Interestingly, this was not the case for juveniles, as acclimated and unacclimated groups did not differ with respect to whole-body Na+ concentrations. The results of this study show that age influences the time course and possibly the mechanisms of acclimation in fathead minnows exposed to Cu.

Original languageEnglish (US)
Pages (from-to)97-109
Number of pages13
JournalAquatic Toxicology
Volume74
Issue number2
DOIs
StatePublished - Aug 30 2005

Fingerprint

Pimephales promelas
Cyprinidae
Acclimatization
acclimation
Larva
Fishes
larvae
fish
larva
minnows
lethal dose
testing
depuration
Water
organisms
dosage
experiment
exposure
water
test

Keywords

  • Acclimation
  • Copper
  • Fathead minnow
  • Organism age
  • Whole-body sodium

ASJC Scopus subject areas

  • Aquatic Science
  • Health, Toxicology and Mutagenesis

Cite this

Acclimation to Cu in fathead minnows : Does age influence the response? / Sellin, Marlo K.; Tate-Boldt, Erik; Kolok, Alan S.

In: Aquatic Toxicology, Vol. 74, No. 2, 30.08.2005, p. 97-109.

Research output: Contribution to journalArticle

Sellin, Marlo K. ; Tate-Boldt, Erik ; Kolok, Alan S. / Acclimation to Cu in fathead minnows : Does age influence the response?. In: Aquatic Toxicology. 2005 ; Vol. 74, No. 2. pp. 97-109.
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abstract = "This study had two primary objectives. The first was to determine if the length of exposure necessary for acclimation to Cu to develop in larval fathead minnows (Pimephales promelas) is different than that for juveniles. The second objective was to determine whether the acclimatory response, as determined by organism survival, is consistent with acclimation as determined by whole-body Na+. Six experiments were conducted: four using larval (<20-d-old) and two using juvenile (<60-d-old) fathead minnows. Within each experiment, fish were allocated to one of four groups: unexposed, continuously exposed, episodically exposed or na{\"i}vely exposed. The continuous group was exposed to a sublethal Cu exposure (125 μg/L) for 8, 12, 16 or 20 d and then subjected to a survival test at a lethal dose (375 μg/L). Fish in the episodic group were exposed to the sublethal dose for either 4 or 8 d, given a depuration period of varying lengths (4-16 d) then subjected to a survival test. Na{\"i}ve minnows were maintained in clean water then given the survival challenge. Results from survival tests show that the larvae acclimate after only a 4-d sublethal exposure to Cu. In contrast, juveniles require a 16-d exposure to acclimate. Once acclimation had developed, there was a strong relationship between larval survival and whole-body Na+. Acclimated larvae maintained whole-body Na+ relative to unexposed fish, while unacclimated larvae did not. Interestingly, this was not the case for juveniles, as acclimated and unacclimated groups did not differ with respect to whole-body Na+ concentrations. The results of this study show that age influences the time course and possibly the mechanisms of acclimation in fathead minnows exposed to Cu.",
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