Abnormal serum concentrations of proteins in Parkinson's disease

Ira L. Goldknopf, Jennifer K. Bryson, Irina Strelets, Silvia Quintero, Essam A. Sheta, Miguel Mosqueda, Helen R. Park, Stanley H. Appel, Holly Shill, Marwan Sabbagh, Bruce Chase, Eric Kaldjian, Katerina Markopoulou

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Blood serum was used to identify protein biomarkers for diagnosis of Parkinson's disease (PD) using analytically validated quantitative 2D-gel electrophoresis, and single variable and multivariate statistics. Using banked samples from a first medical center, we identified 57 specific protein spot biomarkers with disease-specific abnormal levels in serum of patients with PD, Alzheimer's disease, amyotrophic lateral sclerosis and similar neurodegenerative conditions (337 samples), when compared to age-matched normal controls (132 samples). To further assess their clinical usefulness in Parkinson's disease, we obtained prospective newly drawn blood serum samples from a second (56 PD, 30 controls) and third (6 PD, 48 controls) medical center. The protein concentrations of the 57 biomarkers were assessed by 2D-gel electrophoresis. Stepwise linear discriminant analysis selected a combination of 21 of the 57 as optimal to distinguish PD patients from controls. When applied to the samples from the second site, the 21 proteins had sensitivity of 93.3% (52 of 56 PD correctly classified), specificity of 92.9% (28 of 30 controls correctly classified); 15 of 15 patients with mild, 28 of 30 with moderate to severe symptoms, and all of the 6 PD patients from the third site were correctly classified. Eleven of the 21 proteins showed statistically significant abnormal concentrations in patients with mild symptoms, and 14 in patients with moderate-severe symptoms. The protein identities reflect the heterogeneity of Parkinson's disease, and thus may provide the capability of monitoring the blood for a diverse range of PD pathophysiological mechanisms: cellular degeneration, oxidative stress, inflammation, and transport.

Original languageEnglish (US)
Pages (from-to)321-327
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume389
Issue number2
DOIs
Publication statusPublished - Nov 13 2009

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Keywords

  • Biomarkers
  • Diagnosis
  • Neurodegenerative
  • Parkinson's
  • Proteins
  • Serum

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Goldknopf, I. L., Bryson, J. K., Strelets, I., Quintero, S., Sheta, E. A., Mosqueda, M., ... Markopoulou, K. (2009). Abnormal serum concentrations of proteins in Parkinson's disease. Biochemical and Biophysical Research Communications, 389(2), 321-327. https://doi.org/10.1016/j.bbrc.2009.08.150