Abstract

Background Peripheral artery disease (PAD), which affects an estimated 27 million people in Europe and North America, is caused by atherosclerotic plaques that limit blood flow to the legs. Chronic, repeated ischemia in the lower leg muscles of PAD patients is associated with loss of normal myofiber morphology and myofiber degradation. In this study, we tested the hypothesis that myofiber morphometrics of PAD calf muscle are significantly different from normal calf muscle and correlate with reduced calf muscle strength and walking performance. Methods Gastrocnemius biopsies were collected from 154 PAD patients (Fontaine stage II) and 85 control subjects. Morphometric parameters of gastrocnemius fibers were determined and evaluated for associations with walking distances and calf muscle strength. Results Compared with control myofibers, PAD myofiber cross-sectional area, major and minor axes, equivalent diameter, perimeter, solidity, and density were significantly decreased (P < 0.005), whereas roundness was significantly increased (P < 0.005). Myofiber morphometric parameters correlated with walking distances and calf muscle strength. Multiple regression analyses demonstrated myofiber cross-sectional area, roundness, and solidity as the best predictors of calf muscle strength and 6-min walking distance, whereas cross-sectional area was the main predictor of maximum walking distance. Conclusions Myofiber morphometrics of PAD gastrocnemius differ significantly from those of control muscle and predict calf muscle strength and walking distances of the PAD patients. Morphometric parameters of gastrocnemius myofibers may serve as objective criteria for diagnosis, staging, and treatment of PAD.

Original languageEnglish (US)
Pages (from-to)172-179
Number of pages8
JournalJournal of Surgical Research
Volume196
Issue number1
DOIs
StatePublished - Jun 1 2015

Fingerprint

Peripheral Arterial Disease
Extremities
Walking
Muscle Strength
Muscles
Leg
Atherosclerotic Plaques
North America
Ischemia
Regression Analysis
Biopsy

Keywords

  • Claudication
  • Myofiber morphology
  • Walking distance

ASJC Scopus subject areas

  • Surgery

Cite this

Abnormal myofiber morphology and limb dysfunction in claudication. / Koutakis, Panagiotis; Myers, Sara A; Cluff, Kim; Ha, Duy M.; Haynatzki, Gleb; McComb, Rodney D; Uchida, Koji; Miserlis, Dimitrios; Papoutsi, Evlampia; Johanning, Jason M; Casale, George P; Pipinos, Iraklis I.

In: Journal of Surgical Research, Vol. 196, No. 1, 01.06.2015, p. 172-179.

Research output: Contribution to journalArticle

Koutakis, Panagiotis ; Myers, Sara A ; Cluff, Kim ; Ha, Duy M. ; Haynatzki, Gleb ; McComb, Rodney D ; Uchida, Koji ; Miserlis, Dimitrios ; Papoutsi, Evlampia ; Johanning, Jason M ; Casale, George P ; Pipinos, Iraklis I. / Abnormal myofiber morphology and limb dysfunction in claudication. In: Journal of Surgical Research. 2015 ; Vol. 196, No. 1. pp. 172-179.
@article{69fe71811fa34cf28c0d8f43b8593c88,
title = "Abnormal myofiber morphology and limb dysfunction in claudication",
abstract = "Background Peripheral artery disease (PAD), which affects an estimated 27 million people in Europe and North America, is caused by atherosclerotic plaques that limit blood flow to the legs. Chronic, repeated ischemia in the lower leg muscles of PAD patients is associated with loss of normal myofiber morphology and myofiber degradation. In this study, we tested the hypothesis that myofiber morphometrics of PAD calf muscle are significantly different from normal calf muscle and correlate with reduced calf muscle strength and walking performance. Methods Gastrocnemius biopsies were collected from 154 PAD patients (Fontaine stage II) and 85 control subjects. Morphometric parameters of gastrocnemius fibers were determined and evaluated for associations with walking distances and calf muscle strength. Results Compared with control myofibers, PAD myofiber cross-sectional area, major and minor axes, equivalent diameter, perimeter, solidity, and density were significantly decreased (P < 0.005), whereas roundness was significantly increased (P < 0.005). Myofiber morphometric parameters correlated with walking distances and calf muscle strength. Multiple regression analyses demonstrated myofiber cross-sectional area, roundness, and solidity as the best predictors of calf muscle strength and 6-min walking distance, whereas cross-sectional area was the main predictor of maximum walking distance. Conclusions Myofiber morphometrics of PAD gastrocnemius differ significantly from those of control muscle and predict calf muscle strength and walking distances of the PAD patients. Morphometric parameters of gastrocnemius myofibers may serve as objective criteria for diagnosis, staging, and treatment of PAD.",
keywords = "Claudication, Myofiber morphology, Walking distance",
author = "Panagiotis Koutakis and Myers, {Sara A} and Kim Cluff and Ha, {Duy M.} and Gleb Haynatzki and McComb, {Rodney D} and Koji Uchida and Dimitrios Miserlis and Evlampia Papoutsi and Johanning, {Jason M} and Casale, {George P} and Pipinos, {Iraklis I}",
year = "2015",
month = "6",
day = "1",
doi = "10.1016/j.jss.2015.02.011",
language = "English (US)",
volume = "196",
pages = "172--179",
journal = "Journal of Surgical Research",
issn = "0022-4804",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Abnormal myofiber morphology and limb dysfunction in claudication

AU - Koutakis, Panagiotis

AU - Myers, Sara A

AU - Cluff, Kim

AU - Ha, Duy M.

AU - Haynatzki, Gleb

AU - McComb, Rodney D

AU - Uchida, Koji

AU - Miserlis, Dimitrios

AU - Papoutsi, Evlampia

AU - Johanning, Jason M

AU - Casale, George P

AU - Pipinos, Iraklis I

PY - 2015/6/1

Y1 - 2015/6/1

N2 - Background Peripheral artery disease (PAD), which affects an estimated 27 million people in Europe and North America, is caused by atherosclerotic plaques that limit blood flow to the legs. Chronic, repeated ischemia in the lower leg muscles of PAD patients is associated with loss of normal myofiber morphology and myofiber degradation. In this study, we tested the hypothesis that myofiber morphometrics of PAD calf muscle are significantly different from normal calf muscle and correlate with reduced calf muscle strength and walking performance. Methods Gastrocnemius biopsies were collected from 154 PAD patients (Fontaine stage II) and 85 control subjects. Morphometric parameters of gastrocnemius fibers were determined and evaluated for associations with walking distances and calf muscle strength. Results Compared with control myofibers, PAD myofiber cross-sectional area, major and minor axes, equivalent diameter, perimeter, solidity, and density were significantly decreased (P < 0.005), whereas roundness was significantly increased (P < 0.005). Myofiber morphometric parameters correlated with walking distances and calf muscle strength. Multiple regression analyses demonstrated myofiber cross-sectional area, roundness, and solidity as the best predictors of calf muscle strength and 6-min walking distance, whereas cross-sectional area was the main predictor of maximum walking distance. Conclusions Myofiber morphometrics of PAD gastrocnemius differ significantly from those of control muscle and predict calf muscle strength and walking distances of the PAD patients. Morphometric parameters of gastrocnemius myofibers may serve as objective criteria for diagnosis, staging, and treatment of PAD.

AB - Background Peripheral artery disease (PAD), which affects an estimated 27 million people in Europe and North America, is caused by atherosclerotic plaques that limit blood flow to the legs. Chronic, repeated ischemia in the lower leg muscles of PAD patients is associated with loss of normal myofiber morphology and myofiber degradation. In this study, we tested the hypothesis that myofiber morphometrics of PAD calf muscle are significantly different from normal calf muscle and correlate with reduced calf muscle strength and walking performance. Methods Gastrocnemius biopsies were collected from 154 PAD patients (Fontaine stage II) and 85 control subjects. Morphometric parameters of gastrocnemius fibers were determined and evaluated for associations with walking distances and calf muscle strength. Results Compared with control myofibers, PAD myofiber cross-sectional area, major and minor axes, equivalent diameter, perimeter, solidity, and density were significantly decreased (P < 0.005), whereas roundness was significantly increased (P < 0.005). Myofiber morphometric parameters correlated with walking distances and calf muscle strength. Multiple regression analyses demonstrated myofiber cross-sectional area, roundness, and solidity as the best predictors of calf muscle strength and 6-min walking distance, whereas cross-sectional area was the main predictor of maximum walking distance. Conclusions Myofiber morphometrics of PAD gastrocnemius differ significantly from those of control muscle and predict calf muscle strength and walking distances of the PAD patients. Morphometric parameters of gastrocnemius myofibers may serve as objective criteria for diagnosis, staging, and treatment of PAD.

KW - Claudication

KW - Myofiber morphology

KW - Walking distance

UR - http://www.scopus.com/inward/record.url?scp=84929274039&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84929274039&partnerID=8YFLogxK

U2 - 10.1016/j.jss.2015.02.011

DO - 10.1016/j.jss.2015.02.011

M3 - Article

VL - 196

SP - 172

EP - 179

JO - Journal of Surgical Research

JF - Journal of Surgical Research

SN - 0022-4804

IS - 1

ER -