Abnormal morphology of fibrillin microfibrils in fibroblast cultures from patients with neonatal Marfan syndrome

Maurice Godfrey, M. Raghunath, J. Cisler, C. L. Bevins, A. DePaepe, M. Di Rocco, J. Gregoritch, K. Imaizumi, P. Kaplan, Y. Kuroki, M. Silberbach, A. Superti-Furga, M. N. Van Thienen, U. Vetter, B. Steinmann

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Abstract

The Marfan syndrome (MFS) is a connective tissue disorder manifested by variable and pleiotropic features in the skeletal, ocular, and cardiovascular systems. The average life span in MFS is about 35 years. A group with much more severe cardiovascular disease and a mean life span of approximately 1 year also exists. We refer to this latter group as 'neonatal Marfan syndrome' (nMFS). Fibrillin defects are now known to be the cause of MFS and nMFS. Immunofluorescence studies were the first to demonstrate this association. Here we describe immunofluorescence studies in a series of 10 neonates and summarize their salient clinical features. In vitro accumulation of fibrillin reactive fibers was assayed using monoclonal antibodies to fibrillin in hyperconfluent fibroblast cultures. As was previously observed in MFS, fibroblast cultures from nMFS patients showed an apparent decrease in accumulation of immunostainable fibrillin. Significantly, however, the morphology of the immunostained fibrils in the nMFS cultures were abnormal and differed not only from control cultures, but also from those seen in cultures of MFS fibroblasts. The nMFS fibrils appeared short, fragmented, and frayed, characteristics that are not seen in MFS. Both the clinical and fibrillin morphology data provide evidence to suggest a useful subclassification of nMFS in the spectrum of MFS.

Original languageEnglish (US)
Pages (from-to)1414-1421
Number of pages8
JournalAmerican Journal of Pathology
Volume146
Issue number6
StatePublished - Jan 1 1995

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Microfibrils
Marfan Syndrome
Fibroblasts
Fibrillins
Fluorescent Antibody Technique
Cardiovascular System
Connective Tissue

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Godfrey, M., Raghunath, M., Cisler, J., Bevins, C. L., DePaepe, A., Di Rocco, M., ... Steinmann, B. (1995). Abnormal morphology of fibrillin microfibrils in fibroblast cultures from patients with neonatal Marfan syndrome. American Journal of Pathology, 146(6), 1414-1421.

Abnormal morphology of fibrillin microfibrils in fibroblast cultures from patients with neonatal Marfan syndrome. / Godfrey, Maurice; Raghunath, M.; Cisler, J.; Bevins, C. L.; DePaepe, A.; Di Rocco, M.; Gregoritch, J.; Imaizumi, K.; Kaplan, P.; Kuroki, Y.; Silberbach, M.; Superti-Furga, A.; Van Thienen, M. N.; Vetter, U.; Steinmann, B.

In: American Journal of Pathology, Vol. 146, No. 6, 01.01.1995, p. 1414-1421.

Research output: Contribution to journalArticle

Godfrey, M, Raghunath, M, Cisler, J, Bevins, CL, DePaepe, A, Di Rocco, M, Gregoritch, J, Imaizumi, K, Kaplan, P, Kuroki, Y, Silberbach, M, Superti-Furga, A, Van Thienen, MN, Vetter, U & Steinmann, B 1995, 'Abnormal morphology of fibrillin microfibrils in fibroblast cultures from patients with neonatal Marfan syndrome', American Journal of Pathology, vol. 146, no. 6, pp. 1414-1421.
Godfrey, Maurice ; Raghunath, M. ; Cisler, J. ; Bevins, C. L. ; DePaepe, A. ; Di Rocco, M. ; Gregoritch, J. ; Imaizumi, K. ; Kaplan, P. ; Kuroki, Y. ; Silberbach, M. ; Superti-Furga, A. ; Van Thienen, M. N. ; Vetter, U. ; Steinmann, B. / Abnormal morphology of fibrillin microfibrils in fibroblast cultures from patients with neonatal Marfan syndrome. In: American Journal of Pathology. 1995 ; Vol. 146, No. 6. pp. 1414-1421.
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AU - DePaepe, A.

AU - Di Rocco, M.

AU - Gregoritch, J.

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AU - Kuroki, Y.

AU - Silberbach, M.

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