Aberrations of 6q13 mapped to the COL12A1 locus in chondromyxoid fibroma

Taketoshi Yasuda, Jun Nishio, Janos Sumegi, Kayla M. Kapels, Pamela A. Althof, Jeffrey R. Sawyer, John D. Reith, Julia A. Bridge

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Chondromyxoid fibroma, a rare benign bone tumor, may be mistaken for chondrosarcoma. Although cytogenetic studies of chondromyxoid fibroma are few, rearrangements of the long arm of chromosome 6, frequently expressed as an inv(6)(p25q13), are prominent. In this study, conventional cytogenetic analysis of 16 chondromyxoid fibroma samples from 14 patients revealed rearrangements of chromosome 6 in 10 of 11 clonally abnormal specimens. In addition to 6q13 rearrangements, recurrent 6p25 and 6q25 anomalies were detected. Notably, an identical t(6;9)(q25;q22) translocation was identified in two cases, suggesting that it represents a distinct translocation of chondromyxoid fibroma. In an effort to further define the aberrant 6q13 breakpoint and identify the molecular consequences, a fluorescence in situ hybridization (FISH)-based positional cloning strategy on chondromyxoid fibroma abnormal metaphase and interphase cells using a series of bacterial and plasmid artificial chromosome (BAC/PAC) probe combinations spanning a 6.1 Mb region was employed. The breakpoint on 6q13 was located within the COL12A1 gene, a collagen gene purportedly involved in another benign bone tumor, subungual exostosis. The findings of this study expand our knowledge of chromosomal alterations in chondromyxoid fibroma, identify COL12A1 as the likely gene candidate within the recurrent 6q13 breakpoint, and provide an alternative approach for detecting 6q13 anomalies in nondividing cells of chondromyxoid fibroma. The latter could potentially be utilized as an adjunct in diagnostically challenging cases.

Original languageEnglish (US)
Pages (from-to)1499-1506
Number of pages8
JournalModern Pathology
Volume22
Issue number11
DOIs
StatePublished - Nov 1 2009

Fingerprint

Fibroma
Chromosomes, Human, Pair 6
Genes
Bacterial Artificial Chromosomes
Bone and Bones
Chondrosarcoma
Cytogenetic Analysis
Interphase
Metaphase
Fluorescence In Situ Hybridization
Cytogenetics
Organism Cloning
Neoplasms
Plasmids
Collagen

Keywords

  • COL12A1
  • Chondromyxoid fibroma
  • Cytogenetics
  • Fluorescence in situ hybridization

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Yasuda, T., Nishio, J., Sumegi, J., Kapels, K. M., Althof, P. A., Sawyer, J. R., ... Bridge, J. A. (2009). Aberrations of 6q13 mapped to the COL12A1 locus in chondromyxoid fibroma. Modern Pathology, 22(11), 1499-1506. https://doi.org/10.1038/modpathol.2009.101

Aberrations of 6q13 mapped to the COL12A1 locus in chondromyxoid fibroma. / Yasuda, Taketoshi; Nishio, Jun; Sumegi, Janos; Kapels, Kayla M.; Althof, Pamela A.; Sawyer, Jeffrey R.; Reith, John D.; Bridge, Julia A.

In: Modern Pathology, Vol. 22, No. 11, 01.11.2009, p. 1499-1506.

Research output: Contribution to journalArticle

Yasuda, T, Nishio, J, Sumegi, J, Kapels, KM, Althof, PA, Sawyer, JR, Reith, JD & Bridge, JA 2009, 'Aberrations of 6q13 mapped to the COL12A1 locus in chondromyxoid fibroma', Modern Pathology, vol. 22, no. 11, pp. 1499-1506. https://doi.org/10.1038/modpathol.2009.101
Yasuda T, Nishio J, Sumegi J, Kapels KM, Althof PA, Sawyer JR et al. Aberrations of 6q13 mapped to the COL12A1 locus in chondromyxoid fibroma. Modern Pathology. 2009 Nov 1;22(11):1499-1506. https://doi.org/10.1038/modpathol.2009.101
Yasuda, Taketoshi ; Nishio, Jun ; Sumegi, Janos ; Kapels, Kayla M. ; Althof, Pamela A. ; Sawyer, Jeffrey R. ; Reith, John D. ; Bridge, Julia A. / Aberrations of 6q13 mapped to the COL12A1 locus in chondromyxoid fibroma. In: Modern Pathology. 2009 ; Vol. 22, No. 11. pp. 1499-1506.
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abstract = "Chondromyxoid fibroma, a rare benign bone tumor, may be mistaken for chondrosarcoma. Although cytogenetic studies of chondromyxoid fibroma are few, rearrangements of the long arm of chromosome 6, frequently expressed as an inv(6)(p25q13), are prominent. In this study, conventional cytogenetic analysis of 16 chondromyxoid fibroma samples from 14 patients revealed rearrangements of chromosome 6 in 10 of 11 clonally abnormal specimens. In addition to 6q13 rearrangements, recurrent 6p25 and 6q25 anomalies were detected. Notably, an identical t(6;9)(q25;q22) translocation was identified in two cases, suggesting that it represents a distinct translocation of chondromyxoid fibroma. In an effort to further define the aberrant 6q13 breakpoint and identify the molecular consequences, a fluorescence in situ hybridization (FISH)-based positional cloning strategy on chondromyxoid fibroma abnormal metaphase and interphase cells using a series of bacterial and plasmid artificial chromosome (BAC/PAC) probe combinations spanning a 6.1 Mb region was employed. The breakpoint on 6q13 was located within the COL12A1 gene, a collagen gene purportedly involved in another benign bone tumor, subungual exostosis. The findings of this study expand our knowledge of chromosomal alterations in chondromyxoid fibroma, identify COL12A1 as the likely gene candidate within the recurrent 6q13 breakpoint, and provide an alternative approach for detecting 6q13 anomalies in nondividing cells of chondromyxoid fibroma. The latter could potentially be utilized as an adjunct in diagnostically challenging cases.",
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