A virus-encoded potassium ion channel is a structural protein in the chlorovirus Paramecium bursaria chlorella virus 1 virion

Giulia Romani, Adrianna Piotrowski, Stefan Hillmer, James Gurnon, James L Van Etten, Anna Moroni, Gerhard Thiel, Brigitte Hertel

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Abstract

Most chloroviruses encode small K+ channels, which are functional in electrophysiological assays. The experimental finding that initial steps in viral infection exhibit the same sensitivity to channel inhibitors as the viral K+ channels has led to the hypothesis that the channels are structural proteins located in the internal membrane of the virus particles. This hypothesis was questioned recently because proteomic studies failed to detect the channel protein in virions of the prototype chlorovirus Paramecium bursaria chlorella virus 1 (PBCV-1). Here, we used a mAb raised against the functional K+ channel from chlorovirus MA-1D to search for the viral K+ channel in the virus particle. The results showed that the antibody was specific and bound to the tetrameric channel on the extracellular side. The antibody reacted in a virus-specific manner with protein extracts from chloroviruses that encoded channels similar to that from MA-1D. There was no cross-reactivity with chloroviruses that encoded more diverse channels or with a chlorovirus that lacked a K+ channel gene. Together with electron microscopic imaging, which revealed labelling of individual virus particles with the channel antibody, these results establish that the viral particles contain an active K+ channel, presumably located in the lipid membrane that surrounds the DNA in the mature virions.

Original languageEnglish (US)
Pages (from-to)2549-2556
Number of pages8
JournalJournal of General Virology
Volume94
Issue numberPART 11
DOIs
StatePublished - Nov 1 2013

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Paramecium
Chlorella
Potassium Channels
Virion
Viruses
Proteins
Antibodies
Virus Diseases
Membrane Lipids
Proteomics
Electrons
Membranes
DNA
Genes

ASJC Scopus subject areas

  • Virology

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A virus-encoded potassium ion channel is a structural protein in the chlorovirus Paramecium bursaria chlorella virus 1 virion. / Romani, Giulia; Piotrowski, Adrianna; Hillmer, Stefan; Gurnon, James; Van Etten, James L; Moroni, Anna; Thiel, Gerhard; Hertel, Brigitte.

In: Journal of General Virology, Vol. 94, No. PART 11, 01.11.2013, p. 2549-2556.

Research output: Contribution to journalArticle

Romani, Giulia ; Piotrowski, Adrianna ; Hillmer, Stefan ; Gurnon, James ; Van Etten, James L ; Moroni, Anna ; Thiel, Gerhard ; Hertel, Brigitte. / A virus-encoded potassium ion channel is a structural protein in the chlorovirus Paramecium bursaria chlorella virus 1 virion. In: Journal of General Virology. 2013 ; Vol. 94, No. PART 11. pp. 2549-2556.
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