A virulent strain of porcine reproductive and respiratory syndrome virus does not up-regulate interleukin-10 levels in vitro or in vivo

Sakthivel Subramaniam, Jung Hyang Sur, Byungjoon Kwon, Asit K Pattnaik, Fernando A. Osorio

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV) causes reproductive failure and respiratory illness in infected pigs. It has been postulated that the ability of PRRSV to induce the anti-inflammatory cytokine interleukin-10 (IL-10) in macrophages of infected pigs would be important for PRRSV immunopathogenesis, although this property would be variable and might be dependent on the strain. Several strains were reported to induce IL-10 in infected macrophages while others would not. In this study, we analyzed the IL-10 expression during in vitro and in vivo infections by a well-characterized virulent strain of PRRSV, vFL12, which is derived from an infectious clone. Our results showed that the vFL12 strain did not up-regulate IL-10 at mRNA or protein levels in either infected macrophages or dendritic cells in vitro. Furthermore, immunofluorescence staining for IL-10 on tonsil sections of PRRSV-infected pigs did not produce any evidence of IL-10 induction in PRRSV-infected cells or in bystander cells of the lymphoid tissues. Hence, based on these results obtained with a well-characterized highly pathogenic PRRSV strain it may be concluded that the induction of IL-10 release is not a part of the PRRSV virulence mechanisms.

Original languageEnglish (US)
Pages (from-to)415-422
Number of pages8
JournalVirus Research
Volume155
Issue number2
DOIs
StatePublished - Feb 1 2011

Fingerprint

Porcine respiratory and reproductive syndrome virus
Interleukin-10
Up-Regulation
Swine
Macrophages
In Vitro Techniques
Palatine Tonsil
Lymphoid Tissue
Respiratory Insufficiency
Dendritic Cells
Fluorescent Antibody Technique
Virulence
Anti-Inflammatory Agents
Clone Cells
Staining and Labeling
Cytokines
Messenger RNA

Keywords

  • Immunofluorescence
  • Interleukin-10
  • PRRSV
  • VFL12
  • Virulence

ASJC Scopus subject areas

  • Cancer Research
  • Virology
  • Infectious Diseases

Cite this

A virulent strain of porcine reproductive and respiratory syndrome virus does not up-regulate interleukin-10 levels in vitro or in vivo. / Subramaniam, Sakthivel; Sur, Jung Hyang; Kwon, Byungjoon; Pattnaik, Asit K; Osorio, Fernando A.

In: Virus Research, Vol. 155, No. 2, 01.02.2011, p. 415-422.

Research output: Contribution to journalArticle

Subramaniam, Sakthivel ; Sur, Jung Hyang ; Kwon, Byungjoon ; Pattnaik, Asit K ; Osorio, Fernando A. / A virulent strain of porcine reproductive and respiratory syndrome virus does not up-regulate interleukin-10 levels in vitro or in vivo. In: Virus Research. 2011 ; Vol. 155, No. 2. pp. 415-422.
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