A vhs-like activity of bovine herpesvirus-1

S. Hinkley, A. P.N. Ambagala, C. J. Jones, S. Srikumaran

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Bovine herpesvirus 1 (BHV-1) is a major pathogen of cattle, causing significant disease including immunosuppression in infected animals. In vitro, the surface expression of major histocompatibility complex (MHC) class I molecules, crucial for an appropriate anti-viral immune response of the host, is down-regulated by BHV-1 infection. Northern blot analyses revealed that the mRNAs for MHC class I and class II molecules were significantly down-regulated in BHV-1 infected cells, starting as early as 2 h after infection. Furthermore, mRNA expression of the two house keeping genes actin and glyceraldehyde-6-phosphate dehydrogenase (GAPDH) was also repressed after infection. This BHV-1 induced effect on cellular metabolism resembled the virion host shutoff (vhs) activity of herpes simplex virus (HSV). Similar to the HSV vhs activity, the putative BHV-1 vhs activity was not abrogated in cells infected in the presence of actinomycin D (ActD) which suggested that no viral gene expression is required for the vhs function and the putative vhs protein is associated with the virion. Sequence comparison indicated a BHV-1 open reading frame having a 60% similarity to the HSV vhs sequence. This putative BHV-1 open reading frame contained the four conserved regions of the alphaherpesvirus vhs protein. Since an HSV vhs-mutant exhibited less virulence and good immunogenicity, we suggest that a BHV-1 vhs- mutant may hold promising potential as a candidate vaccine.

Original languageEnglish (US)
Pages (from-to)2027-2046
Number of pages20
JournalArchives of Virology
Volume145
Issue number10
DOIs
StatePublished - Nov 15 2000

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Bovine Herpesvirus 1
Virion
Simplexvirus
Major Histocompatibility Complex
Open Reading Frames
Glyceraldehyde
Herpesviridae Infections
Messenger RNA
Viral Genes
Essential Genes
Dactinomycin
Infection
Northern Blotting
Immunosuppression
Virulence
Actins
Oxidoreductases
Proteins
Vaccines
Phosphates

ASJC Scopus subject areas

  • Virology

Cite this

Hinkley, S., Ambagala, A. P. N., Jones, C. J., & Srikumaran, S. (2000). A vhs-like activity of bovine herpesvirus-1. Archives of Virology, 145(10), 2027-2046. https://doi.org/10.1007/s007050070038

A vhs-like activity of bovine herpesvirus-1. / Hinkley, S.; Ambagala, A. P.N.; Jones, C. J.; Srikumaran, S.

In: Archives of Virology, Vol. 145, No. 10, 15.11.2000, p. 2027-2046.

Research output: Contribution to journalArticle

Hinkley, S, Ambagala, APN, Jones, CJ & Srikumaran, S 2000, 'A vhs-like activity of bovine herpesvirus-1', Archives of Virology, vol. 145, no. 10, pp. 2027-2046. https://doi.org/10.1007/s007050070038
Hinkley S, Ambagala APN, Jones CJ, Srikumaran S. A vhs-like activity of bovine herpesvirus-1. Archives of Virology. 2000 Nov 15;145(10):2027-2046. https://doi.org/10.1007/s007050070038
Hinkley, S. ; Ambagala, A. P.N. ; Jones, C. J. ; Srikumaran, S. / A vhs-like activity of bovine herpesvirus-1. In: Archives of Virology. 2000 ; Vol. 145, No. 10. pp. 2027-2046.
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