A vancomycin photoprobe identifies the histidine kinase VanSsc as a vancomycin receptor

Kalinka Koteva, Hee Jeon Hong, Xiao Dong Wang, Ishac Nazi, Donald Hughes, Mike J. Naldrett, Mark J. Buttner, Gerard D. Wright

Research output: Contribution to journalArticle

87 Citations (Scopus)

Abstract

Inducible resistance to the glycopeptide antibiotic vancomycin requires expression of vanH, vanA and vanX, controlled by a two-component regulatory system consisting of a receptor histidine kinase, VanS, and a response regulator, VanR. The identity of the VanS receptor ligand has been debated. Using a synthesized vancomycin photoaffinity probe, we show that vancomycin directly binds Streptomyces coelicolor VanS (VanSsc) and this binding is correlated with resistance and required for vanH, vanA and vanX gene expression.

Original languageEnglish (US)
Pages (from-to)327-329
Number of pages3
JournalNature Chemical Biology
Volume6
Issue number5
DOIs
StatePublished - May 2010

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Vancomycin
Streptomyces coelicolor
Glycopeptides
Anti-Bacterial Agents
Ligands
Gene Expression
Histidine Kinase

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

A vancomycin photoprobe identifies the histidine kinase VanSsc as a vancomycin receptor. / Koteva, Kalinka; Hong, Hee Jeon; Wang, Xiao Dong; Nazi, Ishac; Hughes, Donald; Naldrett, Mike J.; Buttner, Mark J.; Wright, Gerard D.

In: Nature Chemical Biology, Vol. 6, No. 5, 05.2010, p. 327-329.

Research output: Contribution to journalArticle

Koteva, K, Hong, HJ, Wang, XD, Nazi, I, Hughes, D, Naldrett, MJ, Buttner, MJ & Wright, GD 2010, 'A vancomycin photoprobe identifies the histidine kinase VanSsc as a vancomycin receptor', Nature Chemical Biology, vol. 6, no. 5, pp. 327-329. https://doi.org/10.1038/nchembio.350
Koteva, Kalinka ; Hong, Hee Jeon ; Wang, Xiao Dong ; Nazi, Ishac ; Hughes, Donald ; Naldrett, Mike J. ; Buttner, Mark J. ; Wright, Gerard D. / A vancomycin photoprobe identifies the histidine kinase VanSsc as a vancomycin receptor. In: Nature Chemical Biology. 2010 ; Vol. 6, No. 5. pp. 327-329.
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