A tubular EHD1-containing compartment involved in the recycling of major histocompatibility complex class I molecules to the plasma membrane

Steve Caplan, Naava Naslavsky, Lisa M. Hartnell, Robert Lodge, Roman S. Polishchuk, Julie G. Donaldson, Juan S. Bonifacino

Research output: Contribution to journalArticle

223 Citations (Scopus)

Abstract

The Eps15 homology (EH) domain-containing protein, EHD1, has recently been ascribed a role in the recycling of receptors internalized by clathrin-mediated endocytosis. A subset of plasma membrane proteins can undergo internalization by a clathrin-independent pathway regulated by the small GTP-binding protein ADP-ribosylation factor 6 (Arf6). Here, we report that endogenous EHD proteins, as well as transgenic tagged EHD1, are associated with long, membrane-bound tubules containing Arf6. EHD1 appears to induce tubule formation, which requires nucleotide cycling on Arf6 and intact microtubules. Mutations in the N-terminal P-loop domain or deletion of the C-terminal EH domain of EHD1 prevent association of EHD1 with tubules or induction of tubule formation. The EHD1 tubules contain internalized major histocompatibility complex class I (MHC-I) molecules that normally traffic through the Arf6 pathway. Recycling assays show that overexpression of EHD1 enhances MHC-I recycling. These observations suggest an additional function of EHD1 as a tubule-inducing factor in the Arf6 pathway for recycling of plasma membrane proteins internalized by clathrin-independent endocytosis.

Original languageEnglish (US)
Pages (from-to)2557-2567
Number of pages11
JournalEMBO Journal
Volume21
Issue number11
DOIs
StatePublished - Jun 3 2002
Externally publishedYes

Fingerprint

Recycling
Cell membranes
Major Histocompatibility Complex
Clathrin
Cell Membrane
Molecules
Endocytosis
Blood Proteins
Membrane Proteins
Electrohydrodynamics
GTP-Binding Proteins
Microtubules
Assays
Proteins
Nucleotides
ADP-ribosylation factor 6
Association reactions
Membranes
Mutation

Keywords

  • Arf6
  • Clathrin-independent
  • EHD1
  • MHC class I
  • Recycling

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

A tubular EHD1-containing compartment involved in the recycling of major histocompatibility complex class I molecules to the plasma membrane. / Caplan, Steve; Naslavsky, Naava; Hartnell, Lisa M.; Lodge, Robert; Polishchuk, Roman S.; Donaldson, Julie G.; Bonifacino, Juan S.

In: EMBO Journal, Vol. 21, No. 11, 03.06.2002, p. 2557-2567.

Research output: Contribution to journalArticle

Caplan, Steve ; Naslavsky, Naava ; Hartnell, Lisa M. ; Lodge, Robert ; Polishchuk, Roman S. ; Donaldson, Julie G. ; Bonifacino, Juan S. / A tubular EHD1-containing compartment involved in the recycling of major histocompatibility complex class I molecules to the plasma membrane. In: EMBO Journal. 2002 ; Vol. 21, No. 11. pp. 2557-2567.
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