A transient receptor potential-like channel mediates synaptic transmission in rod bipolar cells

Yin Shen, J. Alexander Heimel, Maarten Kamermans, Neal S. Peachey, Ronald G. Gregg, Scott Nawy

Research output: Contribution to journalArticle

140 Citations (Scopus)

Abstract

Onbipolar cells are connected to photoreceptors via a sign-inverting synapse. At this synapse, glutamate binds to a metabotropic receptor which couples to the closure of a cation-selective transduction channel. The molecular identity of both the receptor and the G protein are known, but the identity of the transduction channel has remained elusive. Here, we show that the transduction channel in mouse rod bipolar cells, a subtype of On bipolar cell, is likely to be a member of the TRP family of channels. To evoke a transduction current, the metabotropic receptor antagonist LY341495 was applied to the dendrites of cells that were bathed in a solution containing the mGluR6 agonists L-AP4 or glutamate. The transduction current was suppressed by ruthenium red and the TRPV1 antagonists capsazepine and SB-366791. Furthermore, focal application of the TRPV1 agonists capsaicin and anandamide evoked a transduction-like current. The capsaicin-evoked and endogenous transduction current displayed prominent outward rectification, a property of the TRPV1 channel. To test the possibility that the transduction channel is TRPV1, we measured rod bipolar cell function in the TRPV1-/- mouse. The ERG b-wave, a measure of On bipolar cell function, as well as the transduction current and the response to TRPV1 agonists were normal, arguing against a role for TRPV1. However, ERG measurements from mice lacking TRPM1 receptors, another TRP channel implicated in retinal function, revealed the absence of a b-wave. Our results suggest that a TRP-like channel, possibly TRPM1, is essential for synaptic function in On bipolar cells.

Original languageEnglish (US)
Pages (from-to)6088-6093
Number of pages6
JournalJournal of Neuroscience
Volume29
Issue number19
DOIs
StatePublished - May 13 2009

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Transient Receptor Potential Channels
Synaptic Transmission
Capsaicin
Synapses
Glutamic Acid
LY 341495
Ruthenium Red
Dendrites
GTP-Binding Proteins
Cations

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Shen, Y., Heimel, J. A., Kamermans, M., Peachey, N. S., Gregg, R. G., & Nawy, S. (2009). A transient receptor potential-like channel mediates synaptic transmission in rod bipolar cells. Journal of Neuroscience, 29(19), 6088-6093. https://doi.org/10.1523/JNEUROSCI.0132-09.2009

A transient receptor potential-like channel mediates synaptic transmission in rod bipolar cells. / Shen, Yin; Heimel, J. Alexander; Kamermans, Maarten; Peachey, Neal S.; Gregg, Ronald G.; Nawy, Scott.

In: Journal of Neuroscience, Vol. 29, No. 19, 13.05.2009, p. 6088-6093.

Research output: Contribution to journalArticle

Shen, Y, Heimel, JA, Kamermans, M, Peachey, NS, Gregg, RG & Nawy, S 2009, 'A transient receptor potential-like channel mediates synaptic transmission in rod bipolar cells', Journal of Neuroscience, vol. 29, no. 19, pp. 6088-6093. https://doi.org/10.1523/JNEUROSCI.0132-09.2009
Shen, Yin ; Heimel, J. Alexander ; Kamermans, Maarten ; Peachey, Neal S. ; Gregg, Ronald G. ; Nawy, Scott. / A transient receptor potential-like channel mediates synaptic transmission in rod bipolar cells. In: Journal of Neuroscience. 2009 ; Vol. 29, No. 19. pp. 6088-6093.
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