A systems approach to designing next generation vaccines: Combining α-galactose modified antigens with nanoparticle platforms

Yashdeep Phanse, Brenda R. Carrillo-Conde, Amanda E. Ramer-Tait, Scott Broderick, Chang Sun Kong, Krishna Rajan, Ramon Flick, Robert B. Mandell, Balaji Narasimhan, Michael J. Wannemuehler

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Innovative vaccine platforms are needed to develop effective countermeasures against emerging and re-emerging diseases. These platforms should direct antigen internalization by antigen presenting cells and promote immunogenic responses. This work describes an innovative systems approach combining two novel platforms, αGalactose (αGal)-modification of antigens and amphiphilic polyanhydride nanoparticles as vaccine delivery vehicles, to rationally design vaccine formulations. Regimens comprising soluble αGal-modified antigen and nanoparticle-encapsulated unmodified antigen induced a high titer, high avidity antibody response with broader epitope recognition of antigenic peptides than other regimen. Proliferation of antigen-specific CD4 + T cells was also enhanced compared to a traditional adjuvant. Combining the technology platforms and augmenting immune response studies with peptide arrays and informatics analysis provides a new paradigm for rational, systems-based design of next generation vaccine platforms against emerging and re-emerging pathogens.

Original languageEnglish (US)
Article number3775
JournalScientific reports
Volume4
DOIs
StatePublished - Jan 20 2014

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Systems Analysis
Galactose
Nanoparticles
Vaccines
Antigens
Polyanhydrides
CD4 Antigens
Peptides
Informatics
Antigen-Presenting Cells
Antibody Formation
Epitopes
Technology
T-Lymphocytes

ASJC Scopus subject areas

  • General

Cite this

A systems approach to designing next generation vaccines : Combining α-galactose modified antigens with nanoparticle platforms. / Phanse, Yashdeep; Carrillo-Conde, Brenda R.; Ramer-Tait, Amanda E.; Broderick, Scott; Kong, Chang Sun; Rajan, Krishna; Flick, Ramon; Mandell, Robert B.; Narasimhan, Balaji; Wannemuehler, Michael J.

In: Scientific reports, Vol. 4, 3775, 20.01.2014.

Research output: Contribution to journalArticle

Phanse, Y, Carrillo-Conde, BR, Ramer-Tait, AE, Broderick, S, Kong, CS, Rajan, K, Flick, R, Mandell, RB, Narasimhan, B & Wannemuehler, MJ 2014, 'A systems approach to designing next generation vaccines: Combining α-galactose modified antigens with nanoparticle platforms', Scientific reports, vol. 4, 3775. https://doi.org/10.1038/srep03775
Phanse, Yashdeep ; Carrillo-Conde, Brenda R. ; Ramer-Tait, Amanda E. ; Broderick, Scott ; Kong, Chang Sun ; Rajan, Krishna ; Flick, Ramon ; Mandell, Robert B. ; Narasimhan, Balaji ; Wannemuehler, Michael J. / A systems approach to designing next generation vaccines : Combining α-galactose modified antigens with nanoparticle platforms. In: Scientific reports. 2014 ; Vol. 4.
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