A 19F NMR study of lomefloxacin in human erythrocytes and its interaction with hemoglobin

Sheila R. Knaub, Melanie J. Priston, Martha D Morton, Joseph D. Slechta, David G. Vander Velde, Christopher M. Riley

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

19F NMR spectroscopy of a model fluoroquinolone, lomefloxacin, in an erythrocyte suspension showed separate resonances for the intra- and extra-cellular compartments. The intra-cellular peak revealed significant line broadening of the fluorine signals of lomefloxacin. Line broadening also occurred in the presence of oxyhemoglobin (HbO2), hematin, globin and iron. This evidence indicated that lomefloxacin interacted with these compounds; however, ultrafiltration experiments indicated that there was only weak binding (5%) of lomefloxacin to HbO2. 19F and 31P NMR spectroscopy revealed that lomefloxacin may compete with 2,3-diphosphoglycerate for its binding site on HbO2. An apparent partition coefficient of 1.90 ± 0.15 was observed for lomefloxacin in human erythrocytes, utilizing LC analysis.

Original languageEnglish (US)
Pages (from-to)1225-1233
Number of pages9
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume13
Issue number10
DOIs
StatePublished - Jan 1 1995

Fingerprint

Hemoglobins
Erythrocytes
Nuclear magnetic resonance
Nuclear magnetic resonance spectroscopy
Magnetic Resonance Spectroscopy
2,3-Diphosphoglycerate
Oxyhemoglobins
Hemin
Globins
Fluorine
Fluoroquinolones
Ultrafiltration
lomefloxacin
Suspensions
Iron
Binding Sites
Experiments

Keywords

  • 2,3-Diphosphoglycerate
  • Erythrocyte
  • F NMR
  • Fluoroquinolone
  • Hemoglobin binding
  • Lomefloxacin
  • P NMR

ASJC Scopus subject areas

  • Analytical Chemistry
  • Pharmaceutical Science
  • Drug Discovery
  • Spectroscopy
  • Clinical Biochemistry

Cite this

A 19F NMR study of lomefloxacin in human erythrocytes and its interaction with hemoglobin. / Knaub, Sheila R.; Priston, Melanie J.; Morton, Martha D; Slechta, Joseph D.; Vander Velde, David G.; Riley, Christopher M.

In: Journal of Pharmaceutical and Biomedical Analysis, Vol. 13, No. 10, 01.01.1995, p. 1225-1233.

Research output: Contribution to journalArticle

Knaub, Sheila R. ; Priston, Melanie J. ; Morton, Martha D ; Slechta, Joseph D. ; Vander Velde, David G. ; Riley, Christopher M. / A 19F NMR study of lomefloxacin in human erythrocytes and its interaction with hemoglobin. In: Journal of Pharmaceutical and Biomedical Analysis. 1995 ; Vol. 13, No. 10. pp. 1225-1233.
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AB - 19F NMR spectroscopy of a model fluoroquinolone, lomefloxacin, in an erythrocyte suspension showed separate resonances for the intra- and extra-cellular compartments. The intra-cellular peak revealed significant line broadening of the fluorine signals of lomefloxacin. Line broadening also occurred in the presence of oxyhemoglobin (HbO2), hematin, globin and iron. This evidence indicated that lomefloxacin interacted with these compounds; however, ultrafiltration experiments indicated that there was only weak binding (5%) of lomefloxacin to HbO2. 19F and 31P NMR spectroscopy revealed that lomefloxacin may compete with 2,3-diphosphoglycerate for its binding site on HbO2. An apparent partition coefficient of 1.90 ± 0.15 was observed for lomefloxacin in human erythrocytes, utilizing LC analysis.

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