A sulfur-based transport pathway in Cu+-ATPases

Daniel Mattle, Limei Zhang, Oleg Sitsel, Lotte Thue Pedersen, Maria Rosa Moncelli, Francesco Tadini-Buoninsegni, Pontus Gourdon, Douglas C. Rees, Poul Nissen, Gabriele Meloni

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Cells regulate copper levels tightly to balance the biogenesis and integrity of copper centers in vital enzymes against toxic levels of copper. PIB-type Cu+-ATPases play a central role in copper homeostasis by catalyzing the selective translocation of Cu+ across cellular membranes. Crystal structures of a copper-free Cu+-ATPase are available, but the mechanism of Cu+ recognition, binding, and translocation remains elusive. Through X-ray absorption spectroscopy, ATPase activity assays, and charge transfer measurements on solid-supported membranes using wild-type and mutant forms of the Legionella pneumophila Cu+-ATPase (LpCopA), we identify a sulfur-lined metal transport pathway. Structural analysis indicates that Cu+ is bound at a high-affinity transmembrane-binding site in a trigonal-planar coordination with the Cys residues of the conserved CPC motif of transmembrane segment 4 (C382 and C384) and the conserved Met residue of transmembrane segment 6 (M717 of the MXXXS motif). These residues are also essential for transport. Additionally, the studies indicate essential roles of other conserved intramembranous polar residues in facilitating copper binding to the high-affinity site and subsequent release through the exit pathway. Synopsis This study identifies a copper transport pathway dominated by sulfur-based residues for the Cu+-ATPase LpCopA from Legionella pneumophila thus providing insights into how metal selectivity and transport is achieved in Cu+-ATPases. Cu+ selection and subsequent extrusion involves a single high-affinity transmembrane Cu+ binding site located at transmembrane helices (TM) 4 and 6. The transmembrane translocation conduit is dominated by sulfur-containing residues. Conserved transmembrane polar residues in TM4-6 possess distinct roles in the transport catalytic cycle. This study identifies a copper transport pathway dominated by sulfur-based residues for the Cu+-ATPase LpCopA from Legionella pneumophila thus providing insights into how metal selectivity and transport is achieved in Cu+-ATPases.

Original languageEnglish (US)
Pages (from-to)728-740
Number of pages13
JournalEMBO Reports
Volume16
Issue number6
DOIs
StatePublished - Jun 1 2015

Fingerprint

Sulfur
Adenosine Triphosphatases
Copper
Legionella pneumophila
Metals
X-Ray Absorption Spectroscopy
Binding Sites
Membranes
X ray absorption spectroscopy
Poisons
Structural analysis
Extrusion
Charge transfer
Assays
Homeostasis
Crystal structure
Enzymes

Keywords

  • Cu coordination
  • Cu-ATPase
  • P-type ATPase
  • X-ray absorption spectroscopy
  • charge transfer measurements
  • membrane protein transporter

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Mattle, D., Zhang, L., Sitsel, O., Pedersen, L. T., Moncelli, M. R., Tadini-Buoninsegni, F., ... Meloni, G. (2015). A sulfur-based transport pathway in Cu+-ATPases. EMBO Reports, 16(6), 728-740. https://doi.org/10.15252/embr.201439927

A sulfur-based transport pathway in Cu+-ATPases. / Mattle, Daniel; Zhang, Limei; Sitsel, Oleg; Pedersen, Lotte Thue; Moncelli, Maria Rosa; Tadini-Buoninsegni, Francesco; Gourdon, Pontus; Rees, Douglas C.; Nissen, Poul; Meloni, Gabriele.

In: EMBO Reports, Vol. 16, No. 6, 01.06.2015, p. 728-740.

Research output: Contribution to journalArticle

Mattle, D, Zhang, L, Sitsel, O, Pedersen, LT, Moncelli, MR, Tadini-Buoninsegni, F, Gourdon, P, Rees, DC, Nissen, P & Meloni, G 2015, 'A sulfur-based transport pathway in Cu+-ATPases', EMBO Reports, vol. 16, no. 6, pp. 728-740. https://doi.org/10.15252/embr.201439927
Mattle D, Zhang L, Sitsel O, Pedersen LT, Moncelli MR, Tadini-Buoninsegni F et al. A sulfur-based transport pathway in Cu+-ATPases. EMBO Reports. 2015 Jun 1;16(6):728-740. https://doi.org/10.15252/embr.201439927
Mattle, Daniel ; Zhang, Limei ; Sitsel, Oleg ; Pedersen, Lotte Thue ; Moncelli, Maria Rosa ; Tadini-Buoninsegni, Francesco ; Gourdon, Pontus ; Rees, Douglas C. ; Nissen, Poul ; Meloni, Gabriele. / A sulfur-based transport pathway in Cu+-ATPases. In: EMBO Reports. 2015 ; Vol. 16, No. 6. pp. 728-740.
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AU - Tadini-Buoninsegni, Francesco

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AB - Cells regulate copper levels tightly to balance the biogenesis and integrity of copper centers in vital enzymes against toxic levels of copper. PIB-type Cu+-ATPases play a central role in copper homeostasis by catalyzing the selective translocation of Cu+ across cellular membranes. Crystal structures of a copper-free Cu+-ATPase are available, but the mechanism of Cu+ recognition, binding, and translocation remains elusive. Through X-ray absorption spectroscopy, ATPase activity assays, and charge transfer measurements on solid-supported membranes using wild-type and mutant forms of the Legionella pneumophila Cu+-ATPase (LpCopA), we identify a sulfur-lined metal transport pathway. Structural analysis indicates that Cu+ is bound at a high-affinity transmembrane-binding site in a trigonal-planar coordination with the Cys residues of the conserved CPC motif of transmembrane segment 4 (C382 and C384) and the conserved Met residue of transmembrane segment 6 (M717 of the MXXXS motif). These residues are also essential for transport. Additionally, the studies indicate essential roles of other conserved intramembranous polar residues in facilitating copper binding to the high-affinity site and subsequent release through the exit pathway. Synopsis This study identifies a copper transport pathway dominated by sulfur-based residues for the Cu+-ATPase LpCopA from Legionella pneumophila thus providing insights into how metal selectivity and transport is achieved in Cu+-ATPases. Cu+ selection and subsequent extrusion involves a single high-affinity transmembrane Cu+ binding site located at transmembrane helices (TM) 4 and 6. The transmembrane translocation conduit is dominated by sulfur-containing residues. Conserved transmembrane polar residues in TM4-6 possess distinct roles in the transport catalytic cycle. This study identifies a copper transport pathway dominated by sulfur-based residues for the Cu+-ATPase LpCopA from Legionella pneumophila thus providing insights into how metal selectivity and transport is achieved in Cu+-ATPases.

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