A successful backcross in Trypanosoma brucei

Wendy Gibson, Georgette Kanmogne, Mick Bailey

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Genetic exchange can take place between different strains of Trypanosoma brucei ssp. when they are cotransmitted via the tsetse fly vector, but the mechanism and limits of compatibility between strains are ill-defined as yet. Following the recovery of several hybrid genotypes with single drug resistance from a cross of drug resistant parental strains, we attempted a series of backcrosses and F1 crosses, selecting hybrids by double drug resistance. Of 4 backcrosses, one produced hybrid progeny, the analysis of which is presented here, but none of the 4 F1 crosses produced hybrid progeny. However, among experimental flies from the 8 crosses, although there were large numbers of salivary gland infections, very few consisted of a mixture of parental clones, a prerequisite for mating. In the successful backcross both parents were diploid, but none of the crosses involving triploid clones produced hybrid progeny. The hybrid-secreting fly from the backcross contained a mixture of hybrid and parental clones. The hybrid clones had approximately 3n DNA contents relative to the 2n parental clones and fell into 2 groups with respect to restriction site polymorphisms in kinetoplast DNA maxi-circles. Fingerprinting by random PCR amplification using 8 different arbitrary primers showed minor variation between the hybrid clones.

Original languageEnglish (US)
Pages (from-to)101-110
Number of pages10
JournalMolecular and Biochemical Parasitology
Volume69
Issue number1
DOIs
StatePublished - Jan 1995

Fingerprint

Trypanosoma brucei brucei
Clone Cells
Drug Resistance
Diptera
Kinetoplast DNA
Tsetse Flies
Triploidy
Salivary Glands
Diploidy
Genotype
Polymerase Chain Reaction
DNA
Infection
Pharmaceutical Preparations

Keywords

  • Genetic exchange
  • Transformation
  • Trisomy
  • Trypanosoma brucei

ASJC Scopus subject areas

  • Parasitology
  • Molecular Biology

Cite this

A successful backcross in Trypanosoma brucei. / Gibson, Wendy; Kanmogne, Georgette; Bailey, Mick.

In: Molecular and Biochemical Parasitology, Vol. 69, No. 1, 01.1995, p. 101-110.

Research output: Contribution to journalArticle

Gibson, Wendy ; Kanmogne, Georgette ; Bailey, Mick. / A successful backcross in Trypanosoma brucei. In: Molecular and Biochemical Parasitology. 1995 ; Vol. 69, No. 1. pp. 101-110.
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