A study of the reversibility of helix-coil transition in DNA

M. P. Perelroyzen, V. I. Lyamichev, Yu A. Kalambet, Yuri L Lyubchenko, A. V. Vologodskii

Research output: Contribution to journalArticle

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Abstract

The reversibility of DNA melting has been thoroughly investigated at different ionic strengths. We concentrated on those stages of the process that do not involve a complete separation of the strands of the double helix. The differential melting curves of pBR 322 DNA and a fragment of T7 phage DNA in a buffer containing 0.02M Na+ have been shown to differ substantially from the differential curves of renaturation. Electron-microscopic mapping of pBR 322 DNA at different degrees of unwinding (by a previously elaborated technique) has shown that the irreversibility of melting under real experimental conditions is connected with the stage of forming new helical regions during renaturation. In a buffer containing 0.2M Na+ the melting curves of the DNAs used (pBR 322, a fragment of T7 phage DHA, a fragment of phage λ DNA, a fragment of ØX174 phage DNA) coincide with the renaturation curves, i.e. the process is equilibrium. We have carried out a semi-quantitative analysis of the emergence of irreversibility in the melting of a double helix. The problem of comparing theoretical and experimental melting curves is discussed.

Original languageEnglish (US)
Pages (from-to)4043-4059
Number of pages17
JournalNucleic acids research
Volume9
Issue number16
DOIs
StatePublished - Aug 25 1981

Fingerprint

Reversibility
Helix
Coil
Melting
DNA
Freezing
Bacteriophages
Nucleic Acid Denaturation
Fragment
Bacteriophage T7
Curve
Irreversibility
Buffers
Buffer
Osmolar Concentration
Quantitative Analysis
Ionic strength
Electrons
Electron
Quantitative analysis

ASJC Scopus subject areas

  • Genetics

Cite this

Perelroyzen, M. P., Lyamichev, V. I., Kalambet, Y. A., Lyubchenko, Y. L., & Vologodskii, A. V. (1981). A study of the reversibility of helix-coil transition in DNA. Nucleic acids research, 9(16), 4043-4059. https://doi.org/10.1093/nar/9.16.4043

A study of the reversibility of helix-coil transition in DNA. / Perelroyzen, M. P.; Lyamichev, V. I.; Kalambet, Yu A.; Lyubchenko, Yuri L; Vologodskii, A. V.

In: Nucleic acids research, Vol. 9, No. 16, 25.08.1981, p. 4043-4059.

Research output: Contribution to journalArticle

Perelroyzen, MP, Lyamichev, VI, Kalambet, YA, Lyubchenko, YL & Vologodskii, AV 1981, 'A study of the reversibility of helix-coil transition in DNA', Nucleic acids research, vol. 9, no. 16, pp. 4043-4059. https://doi.org/10.1093/nar/9.16.4043
Perelroyzen MP, Lyamichev VI, Kalambet YA, Lyubchenko YL, Vologodskii AV. A study of the reversibility of helix-coil transition in DNA. Nucleic acids research. 1981 Aug 25;9(16):4043-4059. https://doi.org/10.1093/nar/9.16.4043
Perelroyzen, M. P. ; Lyamichev, V. I. ; Kalambet, Yu A. ; Lyubchenko, Yuri L ; Vologodskii, A. V. / A study of the reversibility of helix-coil transition in DNA. In: Nucleic acids research. 1981 ; Vol. 9, No. 16. pp. 4043-4059.
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