A small viral potassium ion channel with an inherent inward rectification

Denise Eckert, Tobias Schulze, Julian Stahl, Oliver Rauh, James L Van Etten, Brigitte Hertel, Indra Schroeder, Anna Moroni, Gerhard Thiel

Research output: Contribution to journalArticle

Abstract

Some algal viruses have coding sequences for proteins with structural and functional characteristics of pore modules of complex K+ channels. Here we exploit the structural diversity among these channel orthologs to discover new basic principles of structure/function correlates in K+ channels. The analysis of three similar K+ channels with ≤ 86 amino acids (AA) shows that one channel (Kmpv1) generates an ohmic conductance in HEK293 cells while the other two (KmpvSP1, KmpvPL1) exhibit typical features of canonical Kir channels. Like Kir channels, the rectification of the viral channels is a function of the K+ driving force. Reconstitution of KmpvSP1 and KmpvPL1 in planar lipid bilayers showed rapid channel fluctuations only at voltages negative of the K+ reversal voltage. This rectification was maintained in KCl buffer with 1 mM EDTA, which excludes blocking cations as the source of rectification. This means that rectification of the viral channels must be an inherent property of the channel. The structural basis for rectification was investigated by a chimera between rectifying and non-rectifying channels as well as point mutations making the rectifier similar to the ohmic conducting channel. The results of these experiments exclude the pore with pore helix and selectivity filter as playing a role in rectification. The insensitivity of the rectifier to point mutations suggests that tertiary or quaternary structural interactions between the transmembrane domains are responsible for this type of gating.

Original languageEnglish (US)
Pages (from-to)124-135
Number of pages12
JournalChannels (Austin, Tex.)
Volume13
Issue number1
DOIs
StatePublished - Dec 1 2019

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Potassium Channels
Point Mutation
Phycodnaviridae
Lipid bilayers
HEK293 Cells
Lipid Bilayers
Electric potential
Viruses
Edetic Acid
Cations
Buffers
Amino Acids
Proteins
Experiments

Keywords

  • Ba2+ block
  • Kir channels
  • Viral K+ channel
  • inward rectification

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry

Cite this

A small viral potassium ion channel with an inherent inward rectification. / Eckert, Denise; Schulze, Tobias; Stahl, Julian; Rauh, Oliver; Van Etten, James L; Hertel, Brigitte; Schroeder, Indra; Moroni, Anna; Thiel, Gerhard.

In: Channels (Austin, Tex.), Vol. 13, No. 1, 01.12.2019, p. 124-135.

Research output: Contribution to journalArticle

Eckert, D, Schulze, T, Stahl, J, Rauh, O, Van Etten, JL, Hertel, B, Schroeder, I, Moroni, A & Thiel, G 2019, 'A small viral potassium ion channel with an inherent inward rectification', Channels (Austin, Tex.), vol. 13, no. 1, pp. 124-135. https://doi.org/10.1080/19336950.2019.1605813
Eckert, Denise ; Schulze, Tobias ; Stahl, Julian ; Rauh, Oliver ; Van Etten, James L ; Hertel, Brigitte ; Schroeder, Indra ; Moroni, Anna ; Thiel, Gerhard. / A small viral potassium ion channel with an inherent inward rectification. In: Channels (Austin, Tex.). 2019 ; Vol. 13, No. 1. pp. 124-135.
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