A single base mutation at position 2661 in E.coli 23S ribosomal RNA affects the binding of ternary complex to the ribosome

W. E. Tapprich, A. E. Dahlberg

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

A single base substitution mutation from guanine to cytosine was constructed at position 2661 of Escherichia coli 23S rRNA and cloned into the rrnB operon of the multi-copy plasmid pKK3535. The mutant plasmid was transformed into E.coli to determine the effect of the mutation on cell growth as well as the structural and functional properties of the mutant ribosomes in vivo and in vitro. The results show that the mutant ribosomes have a slower elongation rate and an altered affinity for EF-Tu-tRNA-GTP ternary complex. This supports previous findings which indicated that position 2661 is part of a region of 23S rRNA that forms a recognition site for binding of the ternary complex in the ribosomal A site. Combinations of the 2661 mutation with various mutations in ribosomal protein S12 also demonstrate that elements of both ribosomal subunits work in concert to form this binding site.

Original languageEnglish (US)
Pages (from-to)2649-2655
Number of pages7
JournalEMBO Journal
Volume9
Issue number8
StatePublished - Dec 1 1990

Fingerprint

23S Ribosomal RNA
Ribosomes
Escherichia coli
Plasmids
Binding Sites
Peptide Elongation Factor Tu
Mutation
Cytosine
Guanine
Cell growth
Transfer RNA
Guanosine Triphosphate
Elongation
Substitution reactions
rRNA Operon
Ribosome Subunits
Growth

Keywords

  • EF-Tu
  • Mutagenesis
  • Translation
  • tRNA

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

A single base mutation at position 2661 in E.coli 23S ribosomal RNA affects the binding of ternary complex to the ribosome. / Tapprich, W. E.; Dahlberg, A. E.

In: EMBO Journal, Vol. 9, No. 8, 01.12.1990, p. 2649-2655.

Research output: Contribution to journalArticle

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