A simplified procedure for the determination of betaine in liver

A. J. Barak, D. J. Tuma

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

A convenient procedure for the determination of hepatic betaine levels is described. The method takes advantage of ethanol precipitation to rid acidified tissue extracts of interfering substances. Betaine is reacted with potassium triiodide to form betaine periodide, which is selectively precipitated via pH adjustment. The precipitate of betaine periodide is dissolved in ethylene dichloride and measured spectrophotometrically. The method is specific, accurate, and simple and showed recoveries of from 97 to 103% at two different levels of added betaine. The applicability of the method was shown when it was demonstrated that diets containing different amounts of choline influenced levels of hepatic betaine.

Original languageEnglish (US)
Pages (from-to)860-863
Number of pages4
JournalLipids
Volume14
Issue number10
DOIs
StatePublished - Oct 1 1979

Fingerprint

Betaine
betaine
Liver
liver
ethylene dichloride
Tissue Extracts
choline
Nutrition
Choline
Precipitates
Ethanol
methodology
ethanol
potassium
Diet
Recovery
extracts
diet

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry
  • Cell Biology

Cite this

A simplified procedure for the determination of betaine in liver. / Barak, A. J.; Tuma, D. J.

In: Lipids, Vol. 14, No. 10, 01.10.1979, p. 860-863.

Research output: Contribution to journalArticle

Barak, A. J. ; Tuma, D. J. / A simplified procedure for the determination of betaine in liver. In: Lipids. 1979 ; Vol. 14, No. 10. pp. 860-863.
@article{019d63f19fa048888656ac72762426e5,
title = "A simplified procedure for the determination of betaine in liver",
abstract = "A convenient procedure for the determination of hepatic betaine levels is described. The method takes advantage of ethanol precipitation to rid acidified tissue extracts of interfering substances. Betaine is reacted with potassium triiodide to form betaine periodide, which is selectively precipitated via pH adjustment. The precipitate of betaine periodide is dissolved in ethylene dichloride and measured spectrophotometrically. The method is specific, accurate, and simple and showed recoveries of from 97 to 103{\%} at two different levels of added betaine. The applicability of the method was shown when it was demonstrated that diets containing different amounts of choline influenced levels of hepatic betaine.",
author = "Barak, {A. J.} and Tuma, {D. J.}",
year = "1979",
month = "10",
day = "1",
doi = "10.1007/BF02534129",
language = "English (US)",
volume = "14",
pages = "860--863",
journal = "Lipids",
issn = "0024-4201",
publisher = "Springer Verlag",
number = "10",

}

TY - JOUR

T1 - A simplified procedure for the determination of betaine in liver

AU - Barak, A. J.

AU - Tuma, D. J.

PY - 1979/10/1

Y1 - 1979/10/1

N2 - A convenient procedure for the determination of hepatic betaine levels is described. The method takes advantage of ethanol precipitation to rid acidified tissue extracts of interfering substances. Betaine is reacted with potassium triiodide to form betaine periodide, which is selectively precipitated via pH adjustment. The precipitate of betaine periodide is dissolved in ethylene dichloride and measured spectrophotometrically. The method is specific, accurate, and simple and showed recoveries of from 97 to 103% at two different levels of added betaine. The applicability of the method was shown when it was demonstrated that diets containing different amounts of choline influenced levels of hepatic betaine.

AB - A convenient procedure for the determination of hepatic betaine levels is described. The method takes advantage of ethanol precipitation to rid acidified tissue extracts of interfering substances. Betaine is reacted with potassium triiodide to form betaine periodide, which is selectively precipitated via pH adjustment. The precipitate of betaine periodide is dissolved in ethylene dichloride and measured spectrophotometrically. The method is specific, accurate, and simple and showed recoveries of from 97 to 103% at two different levels of added betaine. The applicability of the method was shown when it was demonstrated that diets containing different amounts of choline influenced levels of hepatic betaine.

UR - http://www.scopus.com/inward/record.url?scp=0018633113&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018633113&partnerID=8YFLogxK

U2 - 10.1007/BF02534129

DO - 10.1007/BF02534129

M3 - Article

C2 - 502764

AN - SCOPUS:0018633113

VL - 14

SP - 860

EP - 863

JO - Lipids

JF - Lipids

SN - 0024-4201

IS - 10

ER -