A Simple and Effective Method for Flow Cytometric Study of Lymphoid Malignancies Using Needle Core Biopsy Specimens

Pei Dong Chi, Natalie S. Freed, Laura Wake, Cynthia Page, Lynette M Smith, Catalina Amador, Hina Naushad Qureishi, Timothy Charles Greiner, Scott J. Rodig, Samuel Jay Pirruccello, Ji Yuan, Kai Fu

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1 Citation (Scopus)

Abstract

Objectives: We developed a simple and effective rinsing technique (RT) of needle biopsies to produce cell suspensions for flow cytometry (FCM) and evaluated whether the RT is comparable to the conventional tissue cell suspension (TCS) technique. Methods: We retrieved 93 needle core biopsy cases employing the RT for FCM and 25 needle biopsy cases using TCS for FCM. Results: The diagnostic concordance between the FCM results and the morphologic diagnoses of both groups was compared. The diagnostic concordance was comparable in the RT group (92.6%) to the TCS group (71.4%). Furthermore, the diagnostic concordance in the RT group was associated with number of isolated cells. The diagnostic accuracy increased significantly when the cell number was above 30,000 in the RT group. Conclusions: The RT for FCM not only maximizes the tissue utilization, but also is a simple and effective method to obtain cell suspension as compared to traditional cell suspension technique.

Original languageEnglish (US)
Pages (from-to)637-643
Number of pages7
JournalCytometry Part B - Clinical Cytometry
Volume94
Issue number5
DOIs
StatePublished - Sep 2018

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Large-Core Needle Biopsy
Suspensions
Flow Cytometry
Neoplasms
Needle Biopsy
Cell Count

Keywords

  • flow cytometry
  • lymphoma
  • needle core biopsy
  • pathologic diagnosis

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

Cite this

@article{d13db3326adb44329799a322a2414007,
title = "A Simple and Effective Method for Flow Cytometric Study of Lymphoid Malignancies Using Needle Core Biopsy Specimens",
abstract = "Objectives: We developed a simple and effective rinsing technique (RT) of needle biopsies to produce cell suspensions for flow cytometry (FCM) and evaluated whether the RT is comparable to the conventional tissue cell suspension (TCS) technique. Methods: We retrieved 93 needle core biopsy cases employing the RT for FCM and 25 needle biopsy cases using TCS for FCM. Results: The diagnostic concordance between the FCM results and the morphologic diagnoses of both groups was compared. The diagnostic concordance was comparable in the RT group (92.6{\%}) to the TCS group (71.4{\%}). Furthermore, the diagnostic concordance in the RT group was associated with number of isolated cells. The diagnostic accuracy increased significantly when the cell number was above 30,000 in the RT group. Conclusions: The RT for FCM not only maximizes the tissue utilization, but also is a simple and effective method to obtain cell suspension as compared to traditional cell suspension technique.",
keywords = "flow cytometry, lymphoma, needle core biopsy, pathologic diagnosis",
author = "Chi, {Pei Dong} and Freed, {Natalie S.} and Laura Wake and Cynthia Page and Smith, {Lynette M} and Catalina Amador and {Naushad Qureishi}, Hina and Greiner, {Timothy Charles} and Rodig, {Scott J.} and Pirruccello, {Samuel Jay} and Ji Yuan and Kai Fu",
year = "2018",
month = "9",
doi = "10.1002/cyto.b.21634",
language = "English (US)",
volume = "94",
pages = "637--643",
journal = "Cytometry Part B - Clinical Cytometry",
issn = "1552-4949",
publisher = "Wiley-Liss Inc.",
number = "5",

}

TY - JOUR

T1 - A Simple and Effective Method for Flow Cytometric Study of Lymphoid Malignancies Using Needle Core Biopsy Specimens

AU - Chi, Pei Dong

AU - Freed, Natalie S.

AU - Wake, Laura

AU - Page, Cynthia

AU - Smith, Lynette M

AU - Amador, Catalina

AU - Naushad Qureishi, Hina

AU - Greiner, Timothy Charles

AU - Rodig, Scott J.

AU - Pirruccello, Samuel Jay

AU - Yuan, Ji

AU - Fu, Kai

PY - 2018/9

Y1 - 2018/9

N2 - Objectives: We developed a simple and effective rinsing technique (RT) of needle biopsies to produce cell suspensions for flow cytometry (FCM) and evaluated whether the RT is comparable to the conventional tissue cell suspension (TCS) technique. Methods: We retrieved 93 needle core biopsy cases employing the RT for FCM and 25 needle biopsy cases using TCS for FCM. Results: The diagnostic concordance between the FCM results and the morphologic diagnoses of both groups was compared. The diagnostic concordance was comparable in the RT group (92.6%) to the TCS group (71.4%). Furthermore, the diagnostic concordance in the RT group was associated with number of isolated cells. The diagnostic accuracy increased significantly when the cell number was above 30,000 in the RT group. Conclusions: The RT for FCM not only maximizes the tissue utilization, but also is a simple and effective method to obtain cell suspension as compared to traditional cell suspension technique.

AB - Objectives: We developed a simple and effective rinsing technique (RT) of needle biopsies to produce cell suspensions for flow cytometry (FCM) and evaluated whether the RT is comparable to the conventional tissue cell suspension (TCS) technique. Methods: We retrieved 93 needle core biopsy cases employing the RT for FCM and 25 needle biopsy cases using TCS for FCM. Results: The diagnostic concordance between the FCM results and the morphologic diagnoses of both groups was compared. The diagnostic concordance was comparable in the RT group (92.6%) to the TCS group (71.4%). Furthermore, the diagnostic concordance in the RT group was associated with number of isolated cells. The diagnostic accuracy increased significantly when the cell number was above 30,000 in the RT group. Conclusions: The RT for FCM not only maximizes the tissue utilization, but also is a simple and effective method to obtain cell suspension as compared to traditional cell suspension technique.

KW - flow cytometry

KW - lymphoma

KW - needle core biopsy

KW - pathologic diagnosis

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