A role for surfactant protein D in innate immunity of the human prostate

Rebecca E. Oberley, Kelli L. Goss, Laila Dahmoush, Kevin A. Ault, Erika C. Crouch, Jeanne M. Snyder

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

OBJECTIVES. Surfactant protein D (SP-D) is a member of the collectin family of proteins, which are involved in host defense mechanisms in the lung. In the present study, we found that SP-D is produced in the human prostate where it may play a role in innate immunity. METHODS AND RESULTS. Using reverse- transcriptase PCR and Western blot analysis, we demonstrate that SP-D mRNA and protein are present in human prostate tissue. In situ hybridization and immunohistochemistry revealed that SP-D mRNA and protein are localized in epithelial cells of prostate glands. Prostate glands that are surrounded by inflammatory cells produce increased amounts of SP-D protein. We also show that SP-D inhibits the infection of LNCaP and P69SV40T prostate epithelial cells by Chlamydia tmchomatis in an in vitro infection assay. Furthermore, using truncated human SP-D mutants, we demonstrate that SP-D binds to Chlamydia trachomatis via its carboxy-terminal lectin domains. CONCLUSIONS. Our in vitro studies suggest that SP-D protects the prostate from infection by pathogens. SP-D protein levels are increased at sites of inflammation in the prostate, suggesting SP-D may also contribute more generally to inflammatory regulation in the prostate.

Original languageEnglish (US)
Pages (from-to)241-251
Number of pages11
JournalProstate
Volume65
Issue number3
DOIs
StatePublished - Nov 1 2005

Fingerprint

Pulmonary Surfactant-Associated Protein D
Innate Immunity
Prostate
Proteins
Collectins
Infection
Epithelial Cells
Messenger RNA
Chlamydia
Chlamydia trachomatis
Defense Mechanisms
Reverse Transcriptase Polymerase Chain Reaction
Lectins
In Situ Hybridization

Keywords

  • Chlamydia trachomatis
  • Human
  • Inflammation
  • Innate host defense
  • Reproductive tract
  • Surfactant protein D

ASJC Scopus subject areas

  • Oncology
  • Urology

Cite this

Oberley, R. E., Goss, K. L., Dahmoush, L., Ault, K. A., Crouch, E. C., & Snyder, J. M. (2005). A role for surfactant protein D in innate immunity of the human prostate. Prostate, 65(3), 241-251. https://doi.org/10.1002/pros.20292

A role for surfactant protein D in innate immunity of the human prostate. / Oberley, Rebecca E.; Goss, Kelli L.; Dahmoush, Laila; Ault, Kevin A.; Crouch, Erika C.; Snyder, Jeanne M.

In: Prostate, Vol. 65, No. 3, 01.11.2005, p. 241-251.

Research output: Contribution to journalArticle

Oberley, RE, Goss, KL, Dahmoush, L, Ault, KA, Crouch, EC & Snyder, JM 2005, 'A role for surfactant protein D in innate immunity of the human prostate', Prostate, vol. 65, no. 3, pp. 241-251. https://doi.org/10.1002/pros.20292
Oberley RE, Goss KL, Dahmoush L, Ault KA, Crouch EC, Snyder JM. A role for surfactant protein D in innate immunity of the human prostate. Prostate. 2005 Nov 1;65(3):241-251. https://doi.org/10.1002/pros.20292
Oberley, Rebecca E. ; Goss, Kelli L. ; Dahmoush, Laila ; Ault, Kevin A. ; Crouch, Erika C. ; Snyder, Jeanne M. / A role for surfactant protein D in innate immunity of the human prostate. In: Prostate. 2005 ; Vol. 65, No. 3. pp. 241-251.
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