A role for human MUC4 mucin gene, the ErbB2 ligand, as a target of TGF-β in pancreatic carcinogenesis

Nicolas Jonckheere, Michaël Perrais, Christophe Mariette, Surinder Kumar Batra, Jean Pierre Aubert, Pascal Pigny, Isabelle Van Seuningen

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

MUC4 encodes a large transmembrane mucin that is overexpressed in pancreatic adenocarcinomas. The molecular mechanisms responsible for that altered pattern of expression are unknown. TGF-β, a pleiotropic cytokine, regulates numerous genes involved in pancreatic carcinogenesis via activation of the Smads proteins and MUC4 promoter is rich in Smad-binding elements. Our aim was to study whether the regulation of MUC4 expression by TGF-β in pancreatic cancer cells was strictly dependent on Smad4 activity. Three pancreatic cancer cell lines, CAPAN-1 (MUC4 + /Smad4-), CAPAN-2 (MUC4 + / Smad4 +) and PANC-1 (MUC4-/Smad4 +), were used. By RT-PCR, transfection assays and immunohistochemistry, we show that (i) both MUC4 mRNA and apomucin expression are upregulated by TGF-β, (ii) Smad2 positively cooperates with Smad4 to activate the promoter, (iii) activation of Smad4 by exogenous TGF-β induces Smad4 binding to the promoter, (iv) Smad7 and c-ski both inhibit activation by Smad4. When Smad4 is mutated and inactive, TGF-β activates MUC4 expression via MAPK, PI3K and PKA signaling pathways. Absence of expression in PANC-1 cells is due to histone deacetylation. Altogether, these results indicate that upregulation of MUC4 by TGF-β is restricted to well-differentiated pancreatic cancer cells, and point out a novel mechanism for TGF-β as a key molecule in targeting MUC4 overexpression in pancreatic adenocarcinomas.

Original languageEnglish (US)
Pages (from-to)5729-5738
Number of pages10
JournalOncogene
Volume23
Issue number34
DOIs
StatePublished - Jul 29 2004

Fingerprint

erbB-2 Genes
Mucins
Pancreatic Neoplasms
Carcinogenesis
Ligands
Adenocarcinoma
Phosphatidylinositol 3-Kinases
Histones
Transfection
Up-Regulation
Immunohistochemistry
Cytokines
Cell Line
Polymerase Chain Reaction
Messenger RNA
Genes
Proteins

Keywords

  • Acetylation
  • MUC4
  • Mucin
  • Pancreatic cancer
  • Smad4
  • TGF-β
  • Transcription

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

Cite this

Jonckheere, N., Perrais, M., Mariette, C., Batra, S. K., Aubert, J. P., Pigny, P., & Van Seuningen, I. (2004). A role for human MUC4 mucin gene, the ErbB2 ligand, as a target of TGF-β in pancreatic carcinogenesis. Oncogene, 23(34), 5729-5738. https://doi.org/10.1038/sj.onc.1207769

A role for human MUC4 mucin gene, the ErbB2 ligand, as a target of TGF-β in pancreatic carcinogenesis. / Jonckheere, Nicolas; Perrais, Michaël; Mariette, Christophe; Batra, Surinder Kumar; Aubert, Jean Pierre; Pigny, Pascal; Van Seuningen, Isabelle.

In: Oncogene, Vol. 23, No. 34, 29.07.2004, p. 5729-5738.

Research output: Contribution to journalArticle

Jonckheere, N, Perrais, M, Mariette, C, Batra, SK, Aubert, JP, Pigny, P & Van Seuningen, I 2004, 'A role for human MUC4 mucin gene, the ErbB2 ligand, as a target of TGF-β in pancreatic carcinogenesis', Oncogene, vol. 23, no. 34, pp. 5729-5738. https://doi.org/10.1038/sj.onc.1207769
Jonckheere, Nicolas ; Perrais, Michaël ; Mariette, Christophe ; Batra, Surinder Kumar ; Aubert, Jean Pierre ; Pigny, Pascal ; Van Seuningen, Isabelle. / A role for human MUC4 mucin gene, the ErbB2 ligand, as a target of TGF-β in pancreatic carcinogenesis. In: Oncogene. 2004 ; Vol. 23, No. 34. pp. 5729-5738.
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