A Role for H2B Ubiquitylation in DNA Replication

Kelly M. Trujillo, Mary Ann Osley

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

The monoubiquitylation of histone H2B plays an important role in gene expression by contributing to the regulation of transcription elongation and mRNA processing and export. We explored additional cellular functions of this histone modification by investigating its localization to intergenic regions. H2B ubiquitylation is present in chromatin around origins of DNA replication in budding yeast, and as DNA is replicated its levels are maintained on daughter strands by the Bre1 ubiquitin ligase. In the absence of H2B ubiquitylation, the prereplication complex is formed and activated, but replication fork progression is slowed down and the replisome becomes unstable in the presence of hydroxyurea. H2B ubiquitylation promotes the assembly or stability of nucleosomes on newly replicated DNA, and this function is postulated to contribute to fork progression and replisome stability.

Original languageEnglish (US)
Pages (from-to)734-746
Number of pages13
JournalMolecular Cell
Volume48
Issue number5
DOIs
StatePublished - Dec 14 2012

Fingerprint

Ubiquitination
DNA Replication
Histone Code
Intergenic DNA
Saccharomycetales
Replication Origin
Hydroxyurea
Nucleosomes
DNA
Ligases
Ubiquitin
Histones
Chromatin
Gene Expression
Messenger RNA

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

A Role for H2B Ubiquitylation in DNA Replication. / Trujillo, Kelly M.; Osley, Mary Ann.

In: Molecular Cell, Vol. 48, No. 5, 14.12.2012, p. 734-746.

Research output: Contribution to journalArticle

Trujillo, Kelly M. ; Osley, Mary Ann. / A Role for H2B Ubiquitylation in DNA Replication. In: Molecular Cell. 2012 ; Vol. 48, No. 5. pp. 734-746.
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