A Role for Calcium in Stabilizing Transport Vesicle Coats

Jatinder P. Ahluwalia, Justin D. Topp, Kelly Weirather, Matthew Zimmerman, Mark Stamnes

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Calcium has been implicated in regulating vesicle fusion reactions, but its potential role in regulating other aspects of protein transport, such as vesicle assembly, is largely unexplored. We find that treating cells with the membrane-permeable calcium chelator, 1,2-bis(2-aminophenoxy)ethane-N,N,N′ ,N′-tetraacetic acid tetrakis(acetoxymethyl ester) (BAPTA-AM), leads to a dramatic redistribution of the vesicle coat protein, coatomer, in the cell. We have used the cell-free reconstitution of coat-protomer I (COPI) vesicle assembly to characterize the mechanisms of this redistribution. We find that the recovery of COPI-coated Golgi vesicles is inhibited by the addition of BAPTA to the cell-free vesicle budding assay. When coatomer-coated membranes are incubated in the presence of calcium chelators, the membranes "uncoat," indicating that calcium is necessary for maintaining the integrity of the coat. This uncoating is reversed by the addition of calcium. Interestingly, BAPTA, a calcium chelator with fast binding kinetics, is more potent at uncoating the coatomer-coated membrane than EGTA, suggesting that a calcium transient or a calcium gradient is important for stabilizing COPI vesicle coat. The primary target for the effects of calcium on coatomer recruitment is a step that occurs after ADP-ribosylation factor binding to the membrane. We suggest that a calcium gradient may serve to regulate the timing of vesicle uncoating.

Original languageEnglish (US)
Pages (from-to)34148-34155
Number of pages8
JournalJournal of Biological Chemistry
Volume276
Issue number36
DOIs
StatePublished - Sep 7 2001

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Transport Vesicles
Calcium
Membranes
Protein Subunits
ADP-Ribosylation Factors
Coated Vesicles
Ethane
Egtazic Acid
Capsid Proteins
Protein Transport
Assays
Esters
Fusion reactions
Cell Membrane
Recovery
Kinetics
Acids

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Ahluwalia, J. P., Topp, J. D., Weirather, K., Zimmerman, M., & Stamnes, M. (2001). A Role for Calcium in Stabilizing Transport Vesicle Coats. Journal of Biological Chemistry, 276(36), 34148-34155. https://doi.org/10.1074/jbc.M105398200

A Role for Calcium in Stabilizing Transport Vesicle Coats. / Ahluwalia, Jatinder P.; Topp, Justin D.; Weirather, Kelly; Zimmerman, Matthew; Stamnes, Mark.

In: Journal of Biological Chemistry, Vol. 276, No. 36, 07.09.2001, p. 34148-34155.

Research output: Contribution to journalArticle

Ahluwalia, JP, Topp, JD, Weirather, K, Zimmerman, M & Stamnes, M 2001, 'A Role for Calcium in Stabilizing Transport Vesicle Coats', Journal of Biological Chemistry, vol. 276, no. 36, pp. 34148-34155. https://doi.org/10.1074/jbc.M105398200
Ahluwalia, Jatinder P. ; Topp, Justin D. ; Weirather, Kelly ; Zimmerman, Matthew ; Stamnes, Mark. / A Role for Calcium in Stabilizing Transport Vesicle Coats. In: Journal of Biological Chemistry. 2001 ; Vol. 276, No. 36. pp. 34148-34155.
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