Abstract
Differentiation of oligodendrocyte progenitors into mature oligodendrocytes involves the timely, cell-type specific expression of a number of different genes. Among these, the expression of the myelin basic protein (MBP) gene closely parallels the course of oligodendrocyte differentiation. To understand how transcription of the myelin basic protein gene is controlled, binding to the distal end of the 5′ flanking sequence of the MBP gene was investigated. Specific protein-DNA complexes were localized to an AP-1-like element located between -1230 and -1240. The protein-DNA complexes formed at this site were shown to change as the cells differentiated. In undifferentiated cells two complexes were formed but, as the cells differentiated, binding was nearly completely lost. One of the two complexes was shown to contain a member of the fos family of transcription factors but no jun family members were involved. Mutation of the AP-1-like site resulted in loss of the complex and a change in expression of a reporter construct driven by the mutated promoter sequence. These results demonstrate a role for the AP-1-like site in repression of MBP gene expression in oligodendrocyte progenitor cells.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 85-91 |
| Number of pages | 7 |
| Journal | International Journal of Developmental Neuroscience |
| Volume | 19 |
| Issue number | 1 |
| DOIs | |
| State | Published - Feb 1 2001 |
Fingerprint
Keywords
- AP-1-like site
- Differentiation
- Expression
- Myelin basic protein gene
ASJC Scopus subject areas
- Developmental Neuroscience
- Developmental Biology
Cite this
A role for an AP-1-like site in the expression of the myelin basic protein gene during differentiation. / Miskimins, Robin; Miskimins, W. Keith.
In: International Journal of Developmental Neuroscience, Vol. 19, No. 1, 01.02.2001, p. 85-91.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A role for an AP-1-like site in the expression of the myelin basic protein gene during differentiation
AU - Miskimins, Robin
AU - Miskimins, W. Keith
PY - 2001/2/1
Y1 - 2001/2/1
N2 - Differentiation of oligodendrocyte progenitors into mature oligodendrocytes involves the timely, cell-type specific expression of a number of different genes. Among these, the expression of the myelin basic protein (MBP) gene closely parallels the course of oligodendrocyte differentiation. To understand how transcription of the myelin basic protein gene is controlled, binding to the distal end of the 5′ flanking sequence of the MBP gene was investigated. Specific protein-DNA complexes were localized to an AP-1-like element located between -1230 and -1240. The protein-DNA complexes formed at this site were shown to change as the cells differentiated. In undifferentiated cells two complexes were formed but, as the cells differentiated, binding was nearly completely lost. One of the two complexes was shown to contain a member of the fos family of transcription factors but no jun family members were involved. Mutation of the AP-1-like site resulted in loss of the complex and a change in expression of a reporter construct driven by the mutated promoter sequence. These results demonstrate a role for the AP-1-like site in repression of MBP gene expression in oligodendrocyte progenitor cells.
AB - Differentiation of oligodendrocyte progenitors into mature oligodendrocytes involves the timely, cell-type specific expression of a number of different genes. Among these, the expression of the myelin basic protein (MBP) gene closely parallels the course of oligodendrocyte differentiation. To understand how transcription of the myelin basic protein gene is controlled, binding to the distal end of the 5′ flanking sequence of the MBP gene was investigated. Specific protein-DNA complexes were localized to an AP-1-like element located between -1230 and -1240. The protein-DNA complexes formed at this site were shown to change as the cells differentiated. In undifferentiated cells two complexes were formed but, as the cells differentiated, binding was nearly completely lost. One of the two complexes was shown to contain a member of the fos family of transcription factors but no jun family members were involved. Mutation of the AP-1-like site resulted in loss of the complex and a change in expression of a reporter construct driven by the mutated promoter sequence. These results demonstrate a role for the AP-1-like site in repression of MBP gene expression in oligodendrocyte progenitor cells.
KW - AP-1-like site
KW - Differentiation
KW - Expression
KW - Myelin basic protein gene
UR - http://www.scopus.com/inward/record.url?scp=0035253014&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035253014&partnerID=8YFLogxK
U2 - 10.1016/S0736-5748(00)00066-6
DO - 10.1016/S0736-5748(00)00066-6
M3 - Article
C2 - 11226757
AN - SCOPUS:0035253014
VL - 19
SP - 85
EP - 91
JO - International Journal of Developmental Neuroscience
JF - International Journal of Developmental Neuroscience
SN - 0736-5748
IS - 1
ER -