A rice kinase-protein interaction map

Xiaodong Ding, Todd Richter, Mei Chen, Hiroaki Fujii, Young Su Seo, Mingtang Xie, Xianwu Zheng, Siddhartha Kanrar, Rebecca A. Stevenson, Christopher Dardick, Ying Li, Hao Jiang, Yan Zhang, Fahong Yu, Laura E. Bartley, Mawsheng Chern, Rebecca Bart, Xiuhua Chen, Lihuang Zhu, William G. Farmerie & 5 others Michael Gribskov, Jian Kang Zhu, Michael E Fromm, Pamela C. Ronald, Wen Yuan Song

Research output: Contribution to journalArticle

92 Citations (Scopus)

Abstract

Plants uniquely contain large numbers of protein kinases, and for the vast majority of the 1,429 kinases predicted in the rice (Oryza sativa) genome, little is known of their functions. Genetic approaches often fail to produce observable phenotypes; thus, new strategies are needed to delineate kinase function. We previously developed a cost-effective high-throughput yeast twohybrid system. Using this system, we have generated a protein interaction map of 116 representative rice kinases and 254 of their interacting proteins. Overall, the resulting interaction map supports a large number of known or predicted kinaseprotein interactions from both plants and animals and reveals many new functional insights. Notably, we found a potential widespread role for E3 ubiquitin ligases in pathogen defense signaling mediated by receptor-like kinases, particularly by the kinases that may have evolved from recently expanded kinase subfamilies in rice. We anticipate that the data provided here will serve as a foundation for targeted functional studies in rice and other plants. The application of yeast two-hybrid and TAPtag analyses for large-scale plant protein interaction studies is also discussed.

Original languageEnglish (US)
Pages (from-to)1478-1492
Number of pages15
JournalPlant Physiology
Volume149
Issue number3
DOIs
StatePublished - Mar 1 2009

Fingerprint

Protein Interaction Maps
protein kinases
phosphotransferases (kinases)
Phosphotransferases
rice
Yeasts
yeasts
Plant Proteins
ubiquitin-protein ligase
Ubiquitin-Protein Ligases
plant proteins
Protein Kinases
Oryza
Oryza sativa
proteins
Genome
Phenotype
phenotype
Costs and Cost Analysis
receptors

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

Cite this

Ding, X., Richter, T., Chen, M., Fujii, H., Su Seo, Y., Xie, M., ... Song, W. Y. (2009). A rice kinase-protein interaction map. Plant Physiology, 149(3), 1478-1492. https://doi.org/10.1104/pp.108.128298

A rice kinase-protein interaction map. / Ding, Xiaodong; Richter, Todd; Chen, Mei; Fujii, Hiroaki; Su Seo, Young; Xie, Mingtang; Zheng, Xianwu; Kanrar, Siddhartha; Stevenson, Rebecca A.; Dardick, Christopher; Li, Ying; Jiang, Hao; Zhang, Yan; Yu, Fahong; Bartley, Laura E.; Chern, Mawsheng; Bart, Rebecca; Chen, Xiuhua; Zhu, Lihuang; Farmerie, William G.; Gribskov, Michael; Zhu, Jian Kang; Fromm, Michael E; Ronald, Pamela C.; Song, Wen Yuan.

In: Plant Physiology, Vol. 149, No. 3, 01.03.2009, p. 1478-1492.

Research output: Contribution to journalArticle

Ding, X, Richter, T, Chen, M, Fujii, H, Su Seo, Y, Xie, M, Zheng, X, Kanrar, S, Stevenson, RA, Dardick, C, Li, Y, Jiang, H, Zhang, Y, Yu, F, Bartley, LE, Chern, M, Bart, R, Chen, X, Zhu, L, Farmerie, WG, Gribskov, M, Zhu, JK, Fromm, ME, Ronald, PC & Song, WY 2009, 'A rice kinase-protein interaction map', Plant Physiology, vol. 149, no. 3, pp. 1478-1492. https://doi.org/10.1104/pp.108.128298
Ding X, Richter T, Chen M, Fujii H, Su Seo Y, Xie M et al. A rice kinase-protein interaction map. Plant Physiology. 2009 Mar 1;149(3):1478-1492. https://doi.org/10.1104/pp.108.128298
Ding, Xiaodong ; Richter, Todd ; Chen, Mei ; Fujii, Hiroaki ; Su Seo, Young ; Xie, Mingtang ; Zheng, Xianwu ; Kanrar, Siddhartha ; Stevenson, Rebecca A. ; Dardick, Christopher ; Li, Ying ; Jiang, Hao ; Zhang, Yan ; Yu, Fahong ; Bartley, Laura E. ; Chern, Mawsheng ; Bart, Rebecca ; Chen, Xiuhua ; Zhu, Lihuang ; Farmerie, William G. ; Gribskov, Michael ; Zhu, Jian Kang ; Fromm, Michael E ; Ronald, Pamela C. ; Song, Wen Yuan. / A rice kinase-protein interaction map. In: Plant Physiology. 2009 ; Vol. 149, No. 3. pp. 1478-1492.
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