A reverse transcription-polymerase chain reaction assay for the detection of avian pneumovirus (Colorado strain)

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A reverse transcription-polymerase chain reaction assay was developed for the detection of avian pneumovirus (Colorado strain) (APV-Col). The specific primers were designed from the published sequence of the matrix protein gene of APV-Col. The primers amplified a product of 631 nucleotides from APV-Col. The assay identified only APV-Col and did not react with Newcastle disease virus and infectious bronchitis virus.

Original languageEnglish (US)
Pages (from-to)600-603
Number of pages4
JournalAvian diseases
Volume43
Issue number3
DOIs
StatePublished - Jan 1 1999

Fingerprint

Infectious bronchitis virus
Metapneumovirus
Avian metapneumovirus
Newcastle disease virus
Reverse Transcription
Nucleotides
reverse transcriptase polymerase chain reaction
Polymerase Chain Reaction
assays
Proteins
nucleotides
genes
proteins

Keywords

  • Avian pneumovirus
  • RT-PCR
  • Turkey rhinotracheitis

ASJC Scopus subject areas

  • Food Animals
  • Animal Science and Zoology
  • Immunology and Microbiology(all)

Cite this

A reverse transcription-polymerase chain reaction assay for the detection of avian pneumovirus (Colorado strain). / Ali, A.; Reynolds, D. L.

In: Avian diseases, Vol. 43, No. 3, 01.01.1999, p. 600-603.

Research output: Contribution to journalArticle

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