A replicon trans-packaging system reveals the requirement of nonstructural proteins for the assembly of bovine viral diarrhea virus (BVDV) virion

Delin Liang, Limei Chen, Israrul H. Ansari, Laura H V G Gil, Christina L. Topliff, Clayton L Kelling, Ruben O. Donis

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A selective trans-packaging system was developed to produce and isolate bovine viral diarrhea virus (BVDV) pseudo-particles with complementing reporter replicons and their packaging proteins expressed in trans with recombinant vaccinia virus. The encapsidation of replicon rNS3-5B was dependent not only on the in trans expression of structural proteins C, Erns, E1 and E2, but also the nonstructural proteins, p7 and contiguous precursor NS2-3-4A. Nonstructural p7, NS4B, NS5A or NS5B could be expressed in cis and in trans with precursor NS2-3-4A without significantly affecting virion assembly efficiency. NS2-3-4A was identified as an in trans functional precursor in virion assembly. BVDV genomes with mutant NS5B, which did not undergo active replication, were packaged 5-fold less efficiently than the intact genomes demonstrating the importance of replication in virion packaging. These results suggest that genome replication and assembly are closely associated, consistent with a model in which these two steps are coupled for maximum efficiency.

Original languageEnglish (US)
Pages (from-to)331-340
Number of pages10
JournalVirology
Volume387
Issue number2
DOIs
StatePublished - May 10 2009

Fingerprint

Bovine Viral Diarrhea Viruses
Replicon
Product Packaging
Virion
Genome
Proteins
Vaccinia virus
Protein C

Keywords

  • BVDV
  • Complementation
  • NS2-3-4A precursor
  • Pseudo-particles
  • Replication
  • Trans-packaging
  • Virion assembly

ASJC Scopus subject areas

  • Virology

Cite this

A replicon trans-packaging system reveals the requirement of nonstructural proteins for the assembly of bovine viral diarrhea virus (BVDV) virion. / Liang, Delin; Chen, Limei; Ansari, Israrul H.; Gil, Laura H V G; Topliff, Christina L.; Kelling, Clayton L; Donis, Ruben O.

In: Virology, Vol. 387, No. 2, 10.05.2009, p. 331-340.

Research output: Contribution to journalArticle

Liang, Delin ; Chen, Limei ; Ansari, Israrul H. ; Gil, Laura H V G ; Topliff, Christina L. ; Kelling, Clayton L ; Donis, Ruben O. / A replicon trans-packaging system reveals the requirement of nonstructural proteins for the assembly of bovine viral diarrhea virus (BVDV) virion. In: Virology. 2009 ; Vol. 387, No. 2. pp. 331-340.
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