A rapid spin through oil results in higher cell-associated concentrations of antiretrovirals compared with conventional cell washing

Theodore J. Cory, Lee C. Winchester, Brian L. Robbins, Courtney V. Fletcher

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Background: Determination of cell-associated antiretroviral drug concentrations is necessary for research into reservoirs of HIV. Variation exists in cell-associated drug concentrations among research groups. One cause for this may be washing cells during processing. We explored spinning cells through oil to minimize this variability. Methods & results: Raltegravir, atazanavir, darunavir, efavirenz, lopinavir and ritonavir concentrations were assessed in CEM.ss T cells washed with HBSS and oil-spun cells. Oil-spun cells had significantly higher concentrations for all drugs compared with samples washed with HBSS. Conclusion: The decline in cell-associated drug concentrations with saline washes compared with a single spin through oil shows the utility of a spin through oil. Oil centrifugation results in high cell-associated drug concentrations, and can be done in a fast, efficient manner.

Original languageEnglish (US)
Pages (from-to)1447-1455
Number of pages9
JournalBioanalysis
Volume7
Issue number12
DOIs
StatePublished - Jul 1 2015

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ASJC Scopus subject areas

  • Analytical Chemistry
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Clinical Biochemistry
  • Medical Laboratory Technology

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