A rapid procedure for purification of EcoRI endonuclease

János Sümegi, Danielle Breedveld, paul Hossenlopp, Pierre Chambon

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

A convenient and rapid procedure has been developed to purify restriction endonuclease Eco RI. The method involves sonication of cells at low ionic strength, precipitation of the endonuclease with Polymin P (a polyethyleneimine), elution of the enzyme from the Polymin P precipitate, ammonium sulfate precipitation and chromatography on phosphocellulose. The purified restriction endonuclease is free of exonuclease and other endonucleases.

Original languageEnglish (US)
Pages (from-to)78-85
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume76
Issue number1
DOIs
StatePublished - May 9 1977

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Deoxyribonuclease EcoRI
Endonucleases
DNA Restriction Enzymes
Purification
Polyethyleneimine
Exonucleases
Sonication
Ammonium Sulfate
Chromatography
Ionic strength
Osmolar Concentration
Precipitates
Enzymes
phosphocellulose

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

A rapid procedure for purification of EcoRI endonuclease. / Sümegi, János; Breedveld, Danielle; Hossenlopp, paul; Chambon, Pierre.

In: Biochemical and Biophysical Research Communications, Vol. 76, No. 1, 09.05.1977, p. 78-85.

Research output: Contribution to journalArticle

Sümegi, János ; Breedveld, Danielle ; Hossenlopp, paul ; Chambon, Pierre. / A rapid procedure for purification of EcoRI endonuclease. In: Biochemical and Biophysical Research Communications. 1977 ; Vol. 76, No. 1. pp. 78-85.
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