A rapid and efficient method for enriching mitochondrial DNA from plants

Mackenzie M. Strehle, Emma Purfeerst, Alan C. Christensen

Research output: Contribution to journalArticle

Abstract

Current mitochondrial purification techniques are tedious and protracted due to their emphasis on recovering physiologically active mitochondria. However, for studies that are exclusively interested in isolating mitochondrial DNA (mtDNA) for applications such as PCR and sequencing, respiring mitochondria − and the complex procedures that stem from the need to retain their function − are unnecessary. Still, global DNA extraction methods have proven insufficient for mitochondrial DNA isolation because nuclear mitochondrial DNA segments (NUMTs) pose unique challenges to accurate mtDNA quantification and characterization. We present a rapid and simple extraction technique that maximizes recovery of mitochondrial DNA from plant cells, while minimizing the presence of nuclear DNA. Through real-time PCR, we show that this method provides a significant increase in the enrichment of mitochondrial DNA compared to that of nuclear DNA in both Arabidopsis thaliana and Brassica rapa. This method has important implications for future mitochondrial DNA analyses as it possesses few procedural limitations and minimizes the analytical problems typically associated with mtDNA purification by other techniques.

Original languageEnglish (US)
Pages (from-to)239-242
Number of pages4
JournalMitochondrial DNA Part B: Resources
Volume3
Issue number1
DOIs
StatePublished - Jan 2 2018

Fingerprint

Mitochondrial DNA
DNA
Mitochondria
Brassica rapa
Plant Cells
Arabidopsis
Real-Time Polymerase Chain Reaction
Polymerase Chain Reaction

Keywords

  • Arabidopsis
  • DNA extraction
  • DNA purification
  • qPCR

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

Cite this

A rapid and efficient method for enriching mitochondrial DNA from plants. / Strehle, Mackenzie M.; Purfeerst, Emma; Christensen, Alan C.

In: Mitochondrial DNA Part B: Resources, Vol. 3, No. 1, 02.01.2018, p. 239-242.

Research output: Contribution to journalArticle

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