A putative RNA-binding protein positively regulates salicylic acid-mediated immunity in Arabidopsis

Yiping Qi, Kenichi Tsuda, Anna Joe, Masanao Sato, Le V. Nguyen, Jane Glazebrook, James R. Alfano, Jerry D. Cohen, Fumiaki Katagiri

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

RNA-binding proteins (RBP) can control gene expression at both transcriptional and post-transcriptional levels. Plants respond to pathogen infection with rapid reprogramming of gene expression. However, little is known about how plant RBP function in plant immunity. Here, we describe the involvement of an RBP, Arabidopsis thaliana RNA-binding protein-defense related 1 (AtRBP-DR1; At4g03110), in resistance to the pathogen Pseudomonas syringae pv. tomato DC3000. AtRBP-DR1 loss-of-function mutants showed enhanced susceptibility to P. syringae pv. tomato DC3000. Overexpression of AtRBP-DR1 led to enhanced resistance to P. syringae pv. tomato DC3000 strains and dwarfism. The hypersensitive response triggered by P. syringae pv. tomato DC3000 avrRpt2 was compromised in the Atrbp-dr1 mutant and enhanced in the AtRBP-DR1 overexpression line at early time points. AtRBP-DR1 overexpression lines showed higher mRNA levels of SID2 and PR1, which are salicylic acid (SA) inducible, as well as spontaneous cell death in mature leaves. Consistent with these observations, the SA level was low in the Atrbp-dr1 mutant but high in the overexpression line. The SA-related phenotype in the overexpression line was fully dependent on SID2. Thus, AtRBPDR1 is a positive regulator of SA-mediated immunity, possibly acting on SA signaling-related genes at a post-transcriptional level.

Original languageEnglish (US)
Pages (from-to)1573-1583
Number of pages11
JournalMolecular Plant-Microbe Interactions
Volume23
Issue number12
DOIs
StatePublished - Dec 1 2010

Fingerprint

RNA-binding proteins
RNA-Binding Proteins
Salicylic Acid
Pseudomonas syringae
Pseudomonas syringae pv. tomato
Arabidopsis
salicylic acid
Immunity
Lycopersicon esculentum
immunity
mutants
Plant Immunity
Plant RNA
Gene Expression
Dwarfism
gene expression
Plant Proteins
dwarfing
pathogens
hypersensitive response

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science

Cite this

Qi, Y., Tsuda, K., Joe, A., Sato, M., Nguyen, L. V., Glazebrook, J., ... Katagiri, F. (2010). A putative RNA-binding protein positively regulates salicylic acid-mediated immunity in Arabidopsis. Molecular Plant-Microbe Interactions, 23(12), 1573-1583. https://doi.org/10.1094/MPMI-05-10-0106

A putative RNA-binding protein positively regulates salicylic acid-mediated immunity in Arabidopsis. / Qi, Yiping; Tsuda, Kenichi; Joe, Anna; Sato, Masanao; Nguyen, Le V.; Glazebrook, Jane; Alfano, James R.; Cohen, Jerry D.; Katagiri, Fumiaki.

In: Molecular Plant-Microbe Interactions, Vol. 23, No. 12, 01.12.2010, p. 1573-1583.

Research output: Contribution to journalArticle

Qi, Y, Tsuda, K, Joe, A, Sato, M, Nguyen, LV, Glazebrook, J, Alfano, JR, Cohen, JD & Katagiri, F 2010, 'A putative RNA-binding protein positively regulates salicylic acid-mediated immunity in Arabidopsis', Molecular Plant-Microbe Interactions, vol. 23, no. 12, pp. 1573-1583. https://doi.org/10.1094/MPMI-05-10-0106
Qi, Yiping ; Tsuda, Kenichi ; Joe, Anna ; Sato, Masanao ; Nguyen, Le V. ; Glazebrook, Jane ; Alfano, James R. ; Cohen, Jerry D. ; Katagiri, Fumiaki. / A putative RNA-binding protein positively regulates salicylic acid-mediated immunity in Arabidopsis. In: Molecular Plant-Microbe Interactions. 2010 ; Vol. 23, No. 12. pp. 1573-1583.
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