A protein (ORF2) encoded by the latency-related gene of bovine herpesvirus 1 interacts with Notch1 and Notch3

Aspen Workman, Devis Sinani, Daraporn Pittayakhajonwut, Clinton J Jones

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Like other Alphaherpesvirinae subfamily members, bovine herpesvirus 1 (BHV-1) establishes latency in sensory neurons. The latency-related RNA (LR-RNA) is abundantly expressed in latently infected sensory neurons. An LR mutant virus with stop codons at the amino terminus of the first open reading frame (ORF) in the LR gene (ORF2) does not reactivate from latency, in part because it induces higher levels of apoptosis in infected neurons. ORF2 is not the only viral product expressed during latency, but it is important for the latency reactivation cycle because it inhibits apoptosis. In this study, a yeast 2-hybrid screen revealed that ORF2 interacted with two cellular transcription factors, Notch1 and Notch3. These interactions were confirmed in mouse neuroblastoma cells by confocal microscopy and in an in vitro "pulldown" assay. During reactivation from latency, Notch3 RNA levels in trigeminal ganglia were higher than those during latency, suggesting that Notch family members promote reactivation from latency or that reactivation promotes Notch expression. A plasmid expressing the Notch1 intercellular domain (ICD) stimulated productive infection and promoters that encode the viral transcription factor bICP0. The Notch3 ICD did not stimulate productive infection as efficiently as the Notch1 ICD and had no effect on bICP0 promoter activity. Plasmids expressing the Notch1 ICD or the Notch3 ICD trans-activated a late promoter encoding glycoprotein C. ORF2 reduced the trans-activation potential of Notch1 and Notch3, suggesting that ORF2 interfered with the trans-activation potential of Notch. These studies provide evidence that ORF2, in addition to inhibiting apoptosis, has the potential to promote establishment and maintenance of latency by sequestering cellular transcription factors.

Original languageEnglish (US)
Pages (from-to)2536-2546
Number of pages11
JournalJournal of virology
Volume85
Issue number6
DOIs
StatePublished - Mar 1 2011

Fingerprint

Bovine Herpesvirus 1
Bovine herpesvirus 1
Transcription Factors
apoptosis
transcription factors
sensory neurons
promoter regions
Sensory Receptor Cells
transcriptional activation
Apoptosis
Alphaherpesvirinae
plasmids
Plasmids
RNA
Genes
Trigeminal Ganglion
Proteins
genes
proteins
Terminator Codon

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

A protein (ORF2) encoded by the latency-related gene of bovine herpesvirus 1 interacts with Notch1 and Notch3. / Workman, Aspen; Sinani, Devis; Pittayakhajonwut, Daraporn; Jones, Clinton J.

In: Journal of virology, Vol. 85, No. 6, 01.03.2011, p. 2536-2546.

Research output: Contribution to journalArticle

Workman, Aspen ; Sinani, Devis ; Pittayakhajonwut, Daraporn ; Jones, Clinton J. / A protein (ORF2) encoded by the latency-related gene of bovine herpesvirus 1 interacts with Notch1 and Notch3. In: Journal of virology. 2011 ; Vol. 85, No. 6. pp. 2536-2546.
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