A protein encoded by the bovine herpesvirus 1 latency-related gene interacts with specific cellular regulatory proteins, including CCAAT enhancer binding protein alpha

Florencia Meyer, Sandra Perez, Vicki Geiser, Mark Sintek, Melissa Inman, Clinton Jones

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Following acute infection, bovine herpesvirus 1 establishes latency in sensory neurons of trigeminal ganglia (TG). Reactivation from latency occurs periodically, resulting in the shedding of infectious virus. The latency-related (LR) RNA is abundantly expressed in TG of latently infected calves, and the expression of LR proteins is necessary for dexamethasone-induced reactivation from latency. Previously published studies also identified an alternatively spliced LR transcript which is abundantly expressed in TG at 7 days after infection and has the potential to encode a novel LR fusion protein. Seven days after infection is when extensive viral gene expression is extinguished in TG and latency is established, suggesting that LR gene products influence the establishment of latency. In this study, we used a bacterial two-hybrid assay to identify cellular proteins that interact with the novel LR fusion protein. The LR fusion protein interacts with two proteins that can induce apoptosis (Bid and Cdc42) and with CCAAT enhancer binding protein alpha (C/EBP-α). Additional studies confirmed that the LR fusion protein interacts with human or insect C/EBP-α. C/EBP-α protein expression is induced in TG neurons of infected calves and after dexamethasone-induced reactivation from latency. Wild-type C/EBP-α, but not a DNA binding mutant of C/EBP-α, enhances plaque formation in bovine cells. We hypothesize that interactions between the LR fusion protein and C/EBP-α promote the establishment of latency.

Original languageEnglish (US)
Pages (from-to)59-67
Number of pages9
JournalJournal of virology
Volume81
Issue number1
DOIs
StatePublished - Jan 1 2007

Fingerprint

CCAAT-Enhancer-Binding Protein-alpha
Bovine herpesvirus 1
regulatory proteins
binding proteins
Trigeminal Ganglion
Genes
Proteins
genes
proteins
dexamethasone
Dexamethasone
calves
infection
Infection
Insect Proteins
two hybrid system techniques
Bovine Herpesvirus 1
Virus Shedding
Bovine herpesvirus 1 latency-related protein
sensory neurons

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

A protein encoded by the bovine herpesvirus 1 latency-related gene interacts with specific cellular regulatory proteins, including CCAAT enhancer binding protein alpha. / Meyer, Florencia; Perez, Sandra; Geiser, Vicki; Sintek, Mark; Inman, Melissa; Jones, Clinton.

In: Journal of virology, Vol. 81, No. 1, 01.01.2007, p. 59-67.

Research output: Contribution to journalArticle

Meyer, Florencia ; Perez, Sandra ; Geiser, Vicki ; Sintek, Mark ; Inman, Melissa ; Jones, Clinton. / A protein encoded by the bovine herpesvirus 1 latency-related gene interacts with specific cellular regulatory proteins, including CCAAT enhancer binding protein alpha. In: Journal of virology. 2007 ; Vol. 81, No. 1. pp. 59-67.
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