A predominance of the T cell receptor Vγ2/Vδ2 subset in human Mycobacteria-responsive T cells suggests germline gene encoded recognition

G. Panchamoorthy, J. McLean, R. L. Modlin, C. T. Morita, S. Ishikawa, M. B. Brenner, H. Band

Research output: Contribution to journalArticle

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Abstract

Little is known about the nature of Ag recognition by the TCR-γδ. The recent observation that γδ T cells preferentially recognize mycobacterial Ag provides a model to examine the molecular basis of γδ-TCR recognition. Here, examination of the Mycobacteria-stimulated peripheral blood T cells with TCR-specific mAb revealed a predominance of T cells bearing Vγ2/Vδ2 gene products. PCR cloning and sequence analysis of the TCR chains demonstrated extensive junctional diversity indicating that the response was polyclonal. The marked in vitro γδ T cell response to Mycobacteria was also detected in newborns before encounters with foreign Ag and exclusively involved the same V-gene usage observed in adults. Together, these results suggest that a major mechanism of γδ T cell reactivity involves recognition mediated by germline-encoded segments of the TCR.

Original languageEnglish (US)
Pages (from-to)3360-3369
Number of pages10
JournalJournal of Immunology
Volume147
Issue number10
StatePublished - Jan 1 1991

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Mycobacterium
T-Cell Antigen Receptor
T-Lymphocytes
Genes
Sequence Analysis
Organism Cloning
Blood Cells
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Panchamoorthy, G., McLean, J., Modlin, R. L., Morita, C. T., Ishikawa, S., Brenner, M. B., & Band, H. (1991). A predominance of the T cell receptor Vγ2/Vδ2 subset in human Mycobacteria-responsive T cells suggests germline gene encoded recognition. Journal of Immunology, 147(10), 3360-3369.

A predominance of the T cell receptor Vγ2/Vδ2 subset in human Mycobacteria-responsive T cells suggests germline gene encoded recognition. / Panchamoorthy, G.; McLean, J.; Modlin, R. L.; Morita, C. T.; Ishikawa, S.; Brenner, M. B.; Band, H.

In: Journal of Immunology, Vol. 147, No. 10, 01.01.1991, p. 3360-3369.

Research output: Contribution to journalArticle

Panchamoorthy, G, McLean, J, Modlin, RL, Morita, CT, Ishikawa, S, Brenner, MB & Band, H 1991, 'A predominance of the T cell receptor Vγ2/Vδ2 subset in human Mycobacteria-responsive T cells suggests germline gene encoded recognition', Journal of Immunology, vol. 147, no. 10, pp. 3360-3369.
Panchamoorthy G, McLean J, Modlin RL, Morita CT, Ishikawa S, Brenner MB et al. A predominance of the T cell receptor Vγ2/Vδ2 subset in human Mycobacteria-responsive T cells suggests germline gene encoded recognition. Journal of Immunology. 1991 Jan 1;147(10):3360-3369.
Panchamoorthy, G. ; McLean, J. ; Modlin, R. L. ; Morita, C. T. ; Ishikawa, S. ; Brenner, M. B. ; Band, H. / A predominance of the T cell receptor Vγ2/Vδ2 subset in human Mycobacteria-responsive T cells suggests germline gene encoded recognition. In: Journal of Immunology. 1991 ; Vol. 147, No. 10. pp. 3360-3369.
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