A potential H-DNA element in the MUC1 promoter does not influence transcription

Gurcharan S. Pahwa, L. James Maher, Michael A Hollingsworth

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

A purine/pyrimidine mirror repeat element (M-PMR3) in the MUC1 promoter has been shown to form H-DNA under in vitro conditions. We investigated this element for biological function in the regulation of transcription of this gene. Chloramphenicol acetyltransferase reporter-promoter constructs were prepared in which the mirror repeat element (PMR3) was intact, deleted, or modified, and their activities were evaluated by transient transfection assays into the cell lines Capan-2, PANC1, and HT-29. Deletion or modification of M-PMR3 increased expression of chloramphenicol acetyltransferase activity in MUC1-expressing cells; however, a role for an H-DNA structure in this activity was not supported by the results.

Original languageEnglish (US)
Pages (from-to)26543-26546
Number of pages4
JournalJournal of Biological Chemistry
Volume271
Issue number43
DOIs
StatePublished - Nov 6 1996

Fingerprint

Chloramphenicol O-Acetyltransferase
Transcription
Mirrors
Transfection
Assays
Genes
Cells
Cell Line
triplex DNA
purine
pyrimidine
In Vitro Techniques

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

A potential H-DNA element in the MUC1 promoter does not influence transcription. / Pahwa, Gurcharan S.; James Maher, L.; Hollingsworth, Michael A.

In: Journal of Biological Chemistry, Vol. 271, No. 43, 06.11.1996, p. 26543-26546.

Research output: Contribution to journalArticle

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