A potassium channel protein encoded by chlorella virus PBCV-1

B. Plugge, S. Gazzarrini, M. Nelson, R. Cerana, J. L. Van Etten, C. Derst, D. DiFrancesco, A. Moroni, G. Thiel

Research output: Contribution to journalArticle

128 Citations (Scopus)

Abstract

The large chlorella virus PBCV-1, which contains double-stranded DNA (dsDNA), encodes a 94-codon open reading frame (ORF) that contains a motif resembling the signature sequence of the pore domain of potassium channel proteins. Phylogenetic analyses of the encoded protein, Kcv, indicate a previously unidentified type of potassium channel. The messenger RNA encoded by the ORF leads to functional expression of a potassium-selective conductance in Xenopus laevis oocytes. The channel blockers amantadine and barium, but not cesium, inhibit this conductance, in addition to virus plaque formation. Thus, PBCV-1 encodes the first known viral protein that functions as a potassium-selective channel and is essential in the virus life cycle.

Original languageEnglish (US)
Pages (from-to)1641-1644
Number of pages4
JournalScience
Volume287
Issue number5458
DOIs
StatePublished - Mar 3 2000

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Chlorella
Potassium Channels
Viruses
Open Reading Frames
Amantadine
Cesium
Proteins
Xenopus laevis
Viral Proteins
Barium
Life Cycle Stages
Codon
Oocytes
Potassium
Messenger RNA

ASJC Scopus subject areas

  • General

Cite this

Plugge, B., Gazzarrini, S., Nelson, M., Cerana, R., Van Etten, J. L., Derst, C., ... Thiel, G. (2000). A potassium channel protein encoded by chlorella virus PBCV-1. Science, 287(5458), 1641-1644. https://doi.org/10.1126/science.287.5458.1641

A potassium channel protein encoded by chlorella virus PBCV-1. / Plugge, B.; Gazzarrini, S.; Nelson, M.; Cerana, R.; Van Etten, J. L.; Derst, C.; DiFrancesco, D.; Moroni, A.; Thiel, G.

In: Science, Vol. 287, No. 5458, 03.03.2000, p. 1641-1644.

Research output: Contribution to journalArticle

Plugge, B, Gazzarrini, S, Nelson, M, Cerana, R, Van Etten, JL, Derst, C, DiFrancesco, D, Moroni, A & Thiel, G 2000, 'A potassium channel protein encoded by chlorella virus PBCV-1', Science, vol. 287, no. 5458, pp. 1641-1644. https://doi.org/10.1126/science.287.5458.1641
Plugge B, Gazzarrini S, Nelson M, Cerana R, Van Etten JL, Derst C et al. A potassium channel protein encoded by chlorella virus PBCV-1. Science. 2000 Mar 3;287(5458):1641-1644. https://doi.org/10.1126/science.287.5458.1641
Plugge, B. ; Gazzarrini, S. ; Nelson, M. ; Cerana, R. ; Van Etten, J. L. ; Derst, C. ; DiFrancesco, D. ; Moroni, A. ; Thiel, G. / A potassium channel protein encoded by chlorella virus PBCV-1. In: Science. 2000 ; Vol. 287, No. 5458. pp. 1641-1644.
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