A polymorphic glucocorticoid receptor in a mouse population may explain inherited altered stress response and increased anxiety-type behaviors

Dongsheng Xu, Angela Buehner, Jianping Xu, Travis Lambert, Casey Nekl, Merlyn K Nielsen, You Zhou

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

A polymorphic glucocorticoid receptor (GRQn) with an expanded CAG track and two silent mutations, when compared with the sequence of other isoform (GRwt), is found in two outbred mouse lines that were produced by selection for high (SH) or low (SL) stress response from high or low heat loss lines of mice, respectively. The GRQn allele, which is also found in 5 of 16 commonly used inbred mouse lines, had a much higher frequency in SL mice; the GRwt/wt was found only in the SH line. Both GRQn/Qn and GRwt/Qn mice had a much weaker corticosterone response to stress than the GRwt/wt mice. Assessment of open field activity revealed that GRQn/Qn and GRwt/Qn mice exhibited significantly lower velocity and locomotor activity, less time in the center, and much longer duration in corner zones than the GRwt/wt mice. The increased anxiety-type behaviors of the GRQn/Qn and GR wt/Qn mice were confirmed by the "elevated plus maze" test in which GRQn/Qn and GRwt/Qn mice spent significantly less time in the "open arm" and longer duration in the "closed arm," than GRwt/wt mice. These results suggest this GR polymorphism plays a role in complex mechanisms leading to lower corticosterone response to stress, and may also be associated with decreased locomotive and increased anxiety-type behaviors in mice.

Original languageEnglish (US)
JournalFASEB Journal
Volume20
Issue number13
DOIs
StatePublished - Nov 1 2006

Fingerprint

Glucocorticoid Receptors
Corticosterone
Anxiety
Locomotives
Polymorphism
Heat losses
Population
Protein Isoforms
Locomotion
Hot Temperature
Alleles

Keywords

  • Corticosterone response
  • Elevated plus maze
  • Mouse model
  • Open field assessment
  • Polyglutamine track

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

A polymorphic glucocorticoid receptor in a mouse population may explain inherited altered stress response and increased anxiety-type behaviors. / Xu, Dongsheng; Buehner, Angela; Xu, Jianping; Lambert, Travis; Nekl, Casey; Nielsen, Merlyn K; Zhou, You.

In: FASEB Journal, Vol. 20, No. 13, 01.11.2006.

Research output: Contribution to journalArticle

Xu, Dongsheng ; Buehner, Angela ; Xu, Jianping ; Lambert, Travis ; Nekl, Casey ; Nielsen, Merlyn K ; Zhou, You. / A polymorphic glucocorticoid receptor in a mouse population may explain inherited altered stress response and increased anxiety-type behaviors. In: FASEB Journal. 2006 ; Vol. 20, No. 13.
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