A pneumo-virulent United States isolate of porcine reproductive and respiratory syndrome virus induces apoptosis in bystander cells both in vitro and in vivo

Theerapol Sirinarumitr, Yanjin Zhang, John P. Kluge, Patrick G. Halbur, Prem S. Paul

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

Evidence of apoptosis was detected for the United States porcine reproductive and respiratory syndrome virus (PRRSV) in ATCC CRL11171 cells inoculated with strain ATCC VR2385 and in the tissues of pigs infected with the same strain. Apoptosis was detected by agarose gel electrophoresis, transmission electron microscopy and terminal deoxytransferase dUTP nick end labelling (TUNEL) techniques. By electron microscopy and double-labelling techniques, apoptosis was detected primarily in uninfected bystander cells in the continuous cell line rather than the PRRSV-infected cells. In the lungs, the apoptotic cells were predominantly alveolar and pulmonary intravascular macrophages, and mononuclear cells in the alveolar septa. In the lymph nodes, the apoptotic cells were predominantly tingible body macrophages and mononuclear cells. The induction of apoptosis in a large number of mononuclear cells in the lungs and lymph nodes appears to be a mechanism of PRRSV pathogenesis and might be an explanation for a dramatic reduction in the number of alveolar macrophages and circulating lymphocytes and monocytes in PRRSV-infected pigs.

Original languageEnglish (US)
Pages (from-to)2989-2995
Number of pages7
JournalJournal of General Virology
Volume79
Issue number12
DOIs
StatePublished - Jan 1 1998

Fingerprint

Porcine respiratory and reproductive syndrome virus
Apoptosis
Alveolar Macrophages
Swine
Lymph Nodes
Alveolar Epithelial Cells
Lung
Agar Gel Electrophoresis
Transmission Electron Microscopy
In Vitro Techniques
Monocytes
Electron Microscopy
Cell Count
Macrophages
Lymphocytes
Cell Line

ASJC Scopus subject areas

  • Virology

Cite this

A pneumo-virulent United States isolate of porcine reproductive and respiratory syndrome virus induces apoptosis in bystander cells both in vitro and in vivo. / Sirinarumitr, Theerapol; Zhang, Yanjin; Kluge, John P.; Halbur, Patrick G.; Paul, Prem S.

In: Journal of General Virology, Vol. 79, No. 12, 01.01.1998, p. 2989-2995.

Research output: Contribution to journalArticle

Sirinarumitr, Theerapol ; Zhang, Yanjin ; Kluge, John P. ; Halbur, Patrick G. ; Paul, Prem S. / A pneumo-virulent United States isolate of porcine reproductive and respiratory syndrome virus induces apoptosis in bystander cells both in vitro and in vivo. In: Journal of General Virology. 1998 ; Vol. 79, No. 12. pp. 2989-2995.
@article{12dde04e21c24ae1a4ba88e2cb432c2a,
title = "A pneumo-virulent United States isolate of porcine reproductive and respiratory syndrome virus induces apoptosis in bystander cells both in vitro and in vivo",
abstract = "Evidence of apoptosis was detected for the United States porcine reproductive and respiratory syndrome virus (PRRSV) in ATCC CRL11171 cells inoculated with strain ATCC VR2385 and in the tissues of pigs infected with the same strain. Apoptosis was detected by agarose gel electrophoresis, transmission electron microscopy and terminal deoxytransferase dUTP nick end labelling (TUNEL) techniques. By electron microscopy and double-labelling techniques, apoptosis was detected primarily in uninfected bystander cells in the continuous cell line rather than the PRRSV-infected cells. In the lungs, the apoptotic cells were predominantly alveolar and pulmonary intravascular macrophages, and mononuclear cells in the alveolar septa. In the lymph nodes, the apoptotic cells were predominantly tingible body macrophages and mononuclear cells. The induction of apoptosis in a large number of mononuclear cells in the lungs and lymph nodes appears to be a mechanism of PRRSV pathogenesis and might be an explanation for a dramatic reduction in the number of alveolar macrophages and circulating lymphocytes and monocytes in PRRSV-infected pigs.",
author = "Theerapol Sirinarumitr and Yanjin Zhang and Kluge, {John P.} and Halbur, {Patrick G.} and Paul, {Prem S.}",
year = "1998",
month = "1",
day = "1",
doi = "10.1099/0022-1317-79-12-2989",
language = "English (US)",
volume = "79",
pages = "2989--2995",
journal = "Journal of General Virology",
issn = "0022-1317",
publisher = "Society for General Microbiology",
number = "12",

}

TY - JOUR

T1 - A pneumo-virulent United States isolate of porcine reproductive and respiratory syndrome virus induces apoptosis in bystander cells both in vitro and in vivo

AU - Sirinarumitr, Theerapol

AU - Zhang, Yanjin

AU - Kluge, John P.

AU - Halbur, Patrick G.

AU - Paul, Prem S.

PY - 1998/1/1

Y1 - 1998/1/1

N2 - Evidence of apoptosis was detected for the United States porcine reproductive and respiratory syndrome virus (PRRSV) in ATCC CRL11171 cells inoculated with strain ATCC VR2385 and in the tissues of pigs infected with the same strain. Apoptosis was detected by agarose gel electrophoresis, transmission electron microscopy and terminal deoxytransferase dUTP nick end labelling (TUNEL) techniques. By electron microscopy and double-labelling techniques, apoptosis was detected primarily in uninfected bystander cells in the continuous cell line rather than the PRRSV-infected cells. In the lungs, the apoptotic cells were predominantly alveolar and pulmonary intravascular macrophages, and mononuclear cells in the alveolar septa. In the lymph nodes, the apoptotic cells were predominantly tingible body macrophages and mononuclear cells. The induction of apoptosis in a large number of mononuclear cells in the lungs and lymph nodes appears to be a mechanism of PRRSV pathogenesis and might be an explanation for a dramatic reduction in the number of alveolar macrophages and circulating lymphocytes and monocytes in PRRSV-infected pigs.

AB - Evidence of apoptosis was detected for the United States porcine reproductive and respiratory syndrome virus (PRRSV) in ATCC CRL11171 cells inoculated with strain ATCC VR2385 and in the tissues of pigs infected with the same strain. Apoptosis was detected by agarose gel electrophoresis, transmission electron microscopy and terminal deoxytransferase dUTP nick end labelling (TUNEL) techniques. By electron microscopy and double-labelling techniques, apoptosis was detected primarily in uninfected bystander cells in the continuous cell line rather than the PRRSV-infected cells. In the lungs, the apoptotic cells were predominantly alveolar and pulmonary intravascular macrophages, and mononuclear cells in the alveolar septa. In the lymph nodes, the apoptotic cells were predominantly tingible body macrophages and mononuclear cells. The induction of apoptosis in a large number of mononuclear cells in the lungs and lymph nodes appears to be a mechanism of PRRSV pathogenesis and might be an explanation for a dramatic reduction in the number of alveolar macrophages and circulating lymphocytes and monocytes in PRRSV-infected pigs.

UR - http://www.scopus.com/inward/record.url?scp=0032408195&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032408195&partnerID=8YFLogxK

U2 - 10.1099/0022-1317-79-12-2989

DO - 10.1099/0022-1317-79-12-2989

M3 - Article

C2 - 9880013

AN - SCOPUS:0032408195

VL - 79

SP - 2989

EP - 2995

JO - Journal of General Virology

JF - Journal of General Virology

SN - 0022-1317

IS - 12

ER -