A permease-like protein involved in ER to thylakoid lipid transfer in Arabidopsis

Changcheng Xu, Jilian Fan, Wayne Riekhof, John E. Froehlich, Christoph Benning

Research output: Contribution to journalArticle

146 Citations (Scopus)

Abstract

In eukaryotes, enzymes of different subcellular compartments participate in the assembly of membrane lipids. As a consequence, interorganelle lipid transfer is extensive in growing cells. A prominent example is the transfer of membrane lipid precursors between the endoplasmic reticulum (ER) and the photosynthetic thylakoid membranes in plants. Mono- and digalactolipids are typical photosynthetic membrane lipids. In Arabidopsis, they are derived from one of two pathways, either synthesized de novo in the plastid, or precursors are imported from the ER, giving rise to distinct molecular species. Employing a high-throughput robotic screening procedure generating arrays of spot chromatograms, mutants of Arabidopsis were isolated, which accumulated unusual trigalactolipids. In one allelic mutant subclass, trigalactosyldiacylglycerol1, the primary defect caused a disruption in the biosynthesis of ER-derived thylakoid lipids. Secondarily, a processive galactosyltransferase was activated, leading to the accumulation of oligogalactolipids. Mutations in a permease-like protein of the outer chloroplastic envelope are responsible for the primary biochemical defect. It is proposed that this protein is part of a lipid transfer complex.

Original languageEnglish (US)
Pages (from-to)2370-2379
Number of pages10
JournalEMBO Journal
Volume22
Issue number10
DOIs
StatePublished - May 15 2003

Fingerprint

Thylakoids
Membrane Transport Proteins
Photosynthetic membranes
Membrane Lipids
Galactolipids
Arabidopsis
Endoplasmic Reticulum
Lipids
Galactosyltransferases
Defects
Proteins
Plastids
Biosynthesis
Robotics
Eukaryota
Screening
Throughput
Mutation
Enzymes

Keywords

  • Chloroplast envelopes
  • Lipid arrays
  • Lipid permease
  • Lipid trafficking
  • Thylakoid lipids

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

A permease-like protein involved in ER to thylakoid lipid transfer in Arabidopsis. / Xu, Changcheng; Fan, Jilian; Riekhof, Wayne; Froehlich, John E.; Benning, Christoph.

In: EMBO Journal, Vol. 22, No. 10, 15.05.2003, p. 2370-2379.

Research output: Contribution to journalArticle

Xu, Changcheng ; Fan, Jilian ; Riekhof, Wayne ; Froehlich, John E. ; Benning, Christoph. / A permease-like protein involved in ER to thylakoid lipid transfer in Arabidopsis. In: EMBO Journal. 2003 ; Vol. 22, No. 10. pp. 2370-2379.
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