A novel zinc finger transcription factor linked to salt tolerance in alfalfa

I. Winicov, D. R. Bastola, V. V. Pethe

Research output: Contribution to journalArticle

Abstract

Selection for salt tolerance in alfalfa cell lines is accompanied by constitutive and salt induced changes in mRNA accumulation. The plants regenerated from the salt-tolerant cell lines show heritable salt tolerance through seed, indicating that the cellular mechanisms of salt tolerance also function at the whole plant level. We have cloned and investigated the function of two of these salt-induced transcripts. One of these, (Aff/nî), encodes a putative zinc finger (C4,HC3) transcription factor. Another one, (MsPRP2), encodes a proline rich cell wall protein with a hydrophobic carboxy terminus. Both Alfin 1 and MsPRPZ show salt dependent mRNA accumulation in salt-tolerant culture and roots of the regenerated salt-tolerant plants. To investigate the functions of these genes we have expressed Alfin 1 in bacteria and we have cloned and sequenced the promoter region of MsPRP2. We have demonstrated the DNA binding properties of Alfinl recombinant protein and have cloned the DNA binding sequences by four rounds of selection and PCR amplification. The cloned sequences show a GC rich consensus sequence. Similar sequences are found in promoter fragments of MsPRP2 that show specific binding of recombinant Alfinl. The effect cX Allini over-expression on MsPRP2 is being monitored in transgenic alfalfa.

Original languageEnglish (US)
Pages (from-to)A974
JournalFASEB Journal
Volume11
Issue number9
StatePublished - Dec 1 1997

Fingerprint

Salt-Tolerance
Medicago sativa
zinc finger motif
Zinc Fingers
salt tolerance
Zinc
alfalfa
Salt-Tolerant Plants
Transcription Factors
transcription factors
Salts
salts
GC Rich Sequence
Cell Line
Messenger RNA
Consensus Sequence
Cells
promoter regions
cell lines
Recombinant Proteins

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

A novel zinc finger transcription factor linked to salt tolerance in alfalfa. / Winicov, I.; Bastola, D. R.; Pethe, V. V.

In: FASEB Journal, Vol. 11, No. 9, 01.12.1997, p. A974.

Research output: Contribution to journalArticle

@article{146b7940361744a790fc81e103721cda,
title = "A novel zinc finger transcription factor linked to salt tolerance in alfalfa",
abstract = "Selection for salt tolerance in alfalfa cell lines is accompanied by constitutive and salt induced changes in mRNA accumulation. The plants regenerated from the salt-tolerant cell lines show heritable salt tolerance through seed, indicating that the cellular mechanisms of salt tolerance also function at the whole plant level. We have cloned and investigated the function of two of these salt-induced transcripts. One of these, (Aff/n{\^i}), encodes a putative zinc finger (C4,HC3) transcription factor. Another one, (MsPRP2), encodes a proline rich cell wall protein with a hydrophobic carboxy terminus. Both Alfin 1 and MsPRPZ show salt dependent mRNA accumulation in salt-tolerant culture and roots of the regenerated salt-tolerant plants. To investigate the functions of these genes we have expressed Alfin 1 in bacteria and we have cloned and sequenced the promoter region of MsPRP2. We have demonstrated the DNA binding properties of Alfinl recombinant protein and have cloned the DNA binding sequences by four rounds of selection and PCR amplification. The cloned sequences show a GC rich consensus sequence. Similar sequences are found in promoter fragments of MsPRP2 that show specific binding of recombinant Alfinl. The effect cX Allini over-expression on MsPRP2 is being monitored in transgenic alfalfa.",
author = "I. Winicov and Bastola, {D. R.} and Pethe, {V. V.}",
year = "1997",
month = "12",
day = "1",
language = "English (US)",
volume = "11",
pages = "A974",
journal = "FASEB Journal",
issn = "0892-6638",
publisher = "FASEB",
number = "9",

}

TY - JOUR

T1 - A novel zinc finger transcription factor linked to salt tolerance in alfalfa

AU - Winicov, I.

AU - Bastola, D. R.

AU - Pethe, V. V.

PY - 1997/12/1

Y1 - 1997/12/1

N2 - Selection for salt tolerance in alfalfa cell lines is accompanied by constitutive and salt induced changes in mRNA accumulation. The plants regenerated from the salt-tolerant cell lines show heritable salt tolerance through seed, indicating that the cellular mechanisms of salt tolerance also function at the whole plant level. We have cloned and investigated the function of two of these salt-induced transcripts. One of these, (Aff/nî), encodes a putative zinc finger (C4,HC3) transcription factor. Another one, (MsPRP2), encodes a proline rich cell wall protein with a hydrophobic carboxy terminus. Both Alfin 1 and MsPRPZ show salt dependent mRNA accumulation in salt-tolerant culture and roots of the regenerated salt-tolerant plants. To investigate the functions of these genes we have expressed Alfin 1 in bacteria and we have cloned and sequenced the promoter region of MsPRP2. We have demonstrated the DNA binding properties of Alfinl recombinant protein and have cloned the DNA binding sequences by four rounds of selection and PCR amplification. The cloned sequences show a GC rich consensus sequence. Similar sequences are found in promoter fragments of MsPRP2 that show specific binding of recombinant Alfinl. The effect cX Allini over-expression on MsPRP2 is being monitored in transgenic alfalfa.

AB - Selection for salt tolerance in alfalfa cell lines is accompanied by constitutive and salt induced changes in mRNA accumulation. The plants regenerated from the salt-tolerant cell lines show heritable salt tolerance through seed, indicating that the cellular mechanisms of salt tolerance also function at the whole plant level. We have cloned and investigated the function of two of these salt-induced transcripts. One of these, (Aff/nî), encodes a putative zinc finger (C4,HC3) transcription factor. Another one, (MsPRP2), encodes a proline rich cell wall protein with a hydrophobic carboxy terminus. Both Alfin 1 and MsPRPZ show salt dependent mRNA accumulation in salt-tolerant culture and roots of the regenerated salt-tolerant plants. To investigate the functions of these genes we have expressed Alfin 1 in bacteria and we have cloned and sequenced the promoter region of MsPRP2. We have demonstrated the DNA binding properties of Alfinl recombinant protein and have cloned the DNA binding sequences by four rounds of selection and PCR amplification. The cloned sequences show a GC rich consensus sequence. Similar sequences are found in promoter fragments of MsPRP2 that show specific binding of recombinant Alfinl. The effect cX Allini over-expression on MsPRP2 is being monitored in transgenic alfalfa.

UR - http://www.scopus.com/inward/record.url?scp=33750092767&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33750092767&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:33750092767

VL - 11

SP - A974

JO - FASEB Journal

JF - FASEB Journal

SN - 0892-6638

IS - 9

ER -