A Novel Ribonucleoprotein Complex Defined by Monoclonal Antibodies to NIH 3T3 Cells Transfected with Human Pancreatic Adenocarcinoma DNA

Michael A. Hollingsworth, Michael J. Borowitz, Mary Lou Everett, Richard S. Metzgar

Research output: Contribution to journalArticle

Abstract

Three monoclonal antibodies elicited to NIH 3T3 cells transfected with DNA from a human pancreatic adenocarcinoma cell line recognized a novel ribonucleoprotein complex. Minimally, this ribonucleoprotein complex contained a Mt 240,000 protein (by sodium dodecyl sulfate-polyacrylamide gel electrophoresis) and two RNA species with apparent sizes of 1.5 and 3.0 kilobases (by formaldehyde agarose gel electrophoresis). In addition to a cytoplasmic and nuclear subcellular localization, the RNA antigen was secreted from human tumor cell lines and NIH 3T3 cells transfected with pancreatic tumor DNA (inhibitable by monen-sin) and was apparently not a viral or Mycoplasma contaminant. The ribonucleoprotein antigen was detected in some normal tissues by im-munoperoxidase but was not found in or secreted from in vitro cultured normal human fibroblasts, nontransfected or spontaneously transformed NIH 3T3 cells, or normal peripheral blood leukocytes.

Original languageEnglish (US)
Pages (from-to)6015-6022
Number of pages8
JournalCancer Research
Volume50
Issue number18
StatePublished - Sep 15 1990

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NIH 3T3 Cells
Ribonucleoproteins
Adenocarcinoma
Monoclonal Antibodies
DNA
RNA
Antigens
Agar Gel Electrophoresis
Mycoplasma
Tumor Cell Line
Sodium Dodecyl Sulfate
Formaldehyde
Polyacrylamide Gel Electrophoresis
Leukocytes
Fibroblasts
Cell Line
Neoplasms
Proteins

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

A Novel Ribonucleoprotein Complex Defined by Monoclonal Antibodies to NIH 3T3 Cells Transfected with Human Pancreatic Adenocarcinoma DNA. / Hollingsworth, Michael A.; Borowitz, Michael J.; Everett, Mary Lou; Metzgar, Richard S.

In: Cancer Research, Vol. 50, No. 18, 15.09.1990, p. 6015-6022.

Research output: Contribution to journalArticle

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