A Novel Polymorphism in the Fcγ Receptor IIB (CD32B) Transmembrane Region Alters Receptor Signaling

Xiaoli Li, Jianming Wu, Robert H. Carter, Jeffrey C. Edberg, Kaihong Su, Glinda S. Cooper, Robert P. Kimberly

Research output: Contribution to journalArticle

142 Citations (Scopus)

Abstract

Objective. The low-affinity receptor Fcγ receptor IIb (FcγRIIb), with an immunoreceptor tyrosine-based inhibitory motif (ITIM) in the cytoplasmic domain, down-regulates humoral immune responses and modulates the risk of autoimmunity in animal models. The transmembrane domain of FcγRIIb may also contribute to receptor signaling. Therefore, we investigated the biologic significance of single-nucleotide polymorphisms (SNPs) throughout the coding region. Methods. Discovery of SNPs in FCGR2B was performed by direct cycle sequencing of complementary DNA samples derived by reverse transcriptase-polymerase chain reaction. To assess the biologic significance of the nonsynonymous transmembrane SNP, we studied 3 functions influenced by the FcγRIIb transmembrane domain: tyrosine dephosphorylation of CD19, inhibition of B cell receptor (BCR)-induced calcium response, and modulation of BCR- or anti-Fas-induced apoptosis. Results. The nonsynonymous C-to-T transition in the first cytoplasmic exon, originally reported in the Raji cell line, was not found in either the African-American or the Caucasian population, but a nonsynonymous T-to-C transition at nucleotide 775 in exon 4 of FCGR2B, which changes isoleucine to threonine at residue 187 in the transmembrane domain, was significantly more common in African Americans. Using the FcγRIIb mouse B cell line IIA1.6, we expressed both allelic forms as both full-length and truncated cytoplasmic domain constructs. The FCGR2B-187T allele mediated a higher level of CD19 dephosphorylation (P = 0.029) and a greater degree of inhibition of the calcium response (P = 0.003) when co-engaged with BCR than did FCGR2B-1871, independent of the presence of the ITIM. In contrast, FcγRIIb modulation of BCR-induced and anti-Fas antibody-induced cell death rates were similar in IIA1.6 cells expressing either the 1871 or the 187T allelic form. Conclusion. The differential activity of FCGR2B alleles suggests a novel mechanism of FcγRIIb regulation that may influence the risk of autoimmune disease.

Original languageEnglish (US)
Pages (from-to)3242-3252
Number of pages11
JournalArthritis and rheumatism
Volume48
Issue number11
DOIs
StatePublished - Nov 1 2003

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Fc Receptors
B-Lymphocytes
Single Nucleotide Polymorphism
Tyrosine
African Americans
Exons
Alleles
Calcium-Sensing Receptors
Cell Line
Isoleucine
Threonine
Humoral Immunity
Reverse Transcriptase Polymerase Chain Reaction
Autoimmunity
Autoimmune Diseases
Anti-Idiotypic Antibodies
Cell Death
Down-Regulation
Nucleotides
Animal Models

ASJC Scopus subject areas

  • Immunology and Allergy
  • Rheumatology
  • Immunology
  • Pharmacology (medical)

Cite this

Li, X., Wu, J., Carter, R. H., Edberg, J. C., Su, K., Cooper, G. S., & Kimberly, R. P. (2003). A Novel Polymorphism in the Fcγ Receptor IIB (CD32B) Transmembrane Region Alters Receptor Signaling. Arthritis and rheumatism, 48(11), 3242-3252. https://doi.org/10.1002/art.11313

A Novel Polymorphism in the Fcγ Receptor IIB (CD32B) Transmembrane Region Alters Receptor Signaling. / Li, Xiaoli; Wu, Jianming; Carter, Robert H.; Edberg, Jeffrey C.; Su, Kaihong; Cooper, Glinda S.; Kimberly, Robert P.

In: Arthritis and rheumatism, Vol. 48, No. 11, 01.11.2003, p. 3242-3252.

Research output: Contribution to journalArticle

Li, X, Wu, J, Carter, RH, Edberg, JC, Su, K, Cooper, GS & Kimberly, RP 2003, 'A Novel Polymorphism in the Fcγ Receptor IIB (CD32B) Transmembrane Region Alters Receptor Signaling', Arthritis and rheumatism, vol. 48, no. 11, pp. 3242-3252. https://doi.org/10.1002/art.11313
Li, Xiaoli ; Wu, Jianming ; Carter, Robert H. ; Edberg, Jeffrey C. ; Su, Kaihong ; Cooper, Glinda S. ; Kimberly, Robert P. / A Novel Polymorphism in the Fcγ Receptor IIB (CD32B) Transmembrane Region Alters Receptor Signaling. In: Arthritis and rheumatism. 2003 ; Vol. 48, No. 11. pp. 3242-3252.
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AU - Li, Xiaoli

AU - Wu, Jianming

AU - Carter, Robert H.

AU - Edberg, Jeffrey C.

AU - Su, Kaihong

AU - Cooper, Glinda S.

AU - Kimberly, Robert P.

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N2 - Objective. The low-affinity receptor Fcγ receptor IIb (FcγRIIb), with an immunoreceptor tyrosine-based inhibitory motif (ITIM) in the cytoplasmic domain, down-regulates humoral immune responses and modulates the risk of autoimmunity in animal models. The transmembrane domain of FcγRIIb may also contribute to receptor signaling. Therefore, we investigated the biologic significance of single-nucleotide polymorphisms (SNPs) throughout the coding region. Methods. Discovery of SNPs in FCGR2B was performed by direct cycle sequencing of complementary DNA samples derived by reverse transcriptase-polymerase chain reaction. To assess the biologic significance of the nonsynonymous transmembrane SNP, we studied 3 functions influenced by the FcγRIIb transmembrane domain: tyrosine dephosphorylation of CD19, inhibition of B cell receptor (BCR)-induced calcium response, and modulation of BCR- or anti-Fas-induced apoptosis. Results. The nonsynonymous C-to-T transition in the first cytoplasmic exon, originally reported in the Raji cell line, was not found in either the African-American or the Caucasian population, but a nonsynonymous T-to-C transition at nucleotide 775 in exon 4 of FCGR2B, which changes isoleucine to threonine at residue 187 in the transmembrane domain, was significantly more common in African Americans. Using the FcγRIIb mouse B cell line IIA1.6, we expressed both allelic forms as both full-length and truncated cytoplasmic domain constructs. The FCGR2B-187T allele mediated a higher level of CD19 dephosphorylation (P = 0.029) and a greater degree of inhibition of the calcium response (P = 0.003) when co-engaged with BCR than did FCGR2B-1871, independent of the presence of the ITIM. In contrast, FcγRIIb modulation of BCR-induced and anti-Fas antibody-induced cell death rates were similar in IIA1.6 cells expressing either the 1871 or the 187T allelic form. Conclusion. The differential activity of FCGR2B alleles suggests a novel mechanism of FcγRIIb regulation that may influence the risk of autoimmune disease.

AB - Objective. The low-affinity receptor Fcγ receptor IIb (FcγRIIb), with an immunoreceptor tyrosine-based inhibitory motif (ITIM) in the cytoplasmic domain, down-regulates humoral immune responses and modulates the risk of autoimmunity in animal models. The transmembrane domain of FcγRIIb may also contribute to receptor signaling. Therefore, we investigated the biologic significance of single-nucleotide polymorphisms (SNPs) throughout the coding region. Methods. Discovery of SNPs in FCGR2B was performed by direct cycle sequencing of complementary DNA samples derived by reverse transcriptase-polymerase chain reaction. To assess the biologic significance of the nonsynonymous transmembrane SNP, we studied 3 functions influenced by the FcγRIIb transmembrane domain: tyrosine dephosphorylation of CD19, inhibition of B cell receptor (BCR)-induced calcium response, and modulation of BCR- or anti-Fas-induced apoptosis. Results. The nonsynonymous C-to-T transition in the first cytoplasmic exon, originally reported in the Raji cell line, was not found in either the African-American or the Caucasian population, but a nonsynonymous T-to-C transition at nucleotide 775 in exon 4 of FCGR2B, which changes isoleucine to threonine at residue 187 in the transmembrane domain, was significantly more common in African Americans. Using the FcγRIIb mouse B cell line IIA1.6, we expressed both allelic forms as both full-length and truncated cytoplasmic domain constructs. The FCGR2B-187T allele mediated a higher level of CD19 dephosphorylation (P = 0.029) and a greater degree of inhibition of the calcium response (P = 0.003) when co-engaged with BCR than did FCGR2B-1871, independent of the presence of the ITIM. In contrast, FcγRIIb modulation of BCR-induced and anti-Fas antibody-induced cell death rates were similar in IIA1.6 cells expressing either the 1871 or the 187T allelic form. Conclusion. The differential activity of FCGR2B alleles suggests a novel mechanism of FcγRIIb regulation that may influence the risk of autoimmune disease.

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