A novel pH-sensitive gelatin-DNA semi-interpenetrating polymer network hydrogel

Wen Guang Liu, Xiaowei Li, Gui Xiang Ye, Shu Jun Sun, Dunwan Zhu, Kang De Yao

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Gelatin and DNA were mixed together in various ratios followed by the addition of glutaraldehyde as a cross-linker. FT-IR spectroscopy confirmed the formation of a semi-interpenetrating polymer network (semi-IPN) between the gelatin and DNA. The gelatin-DNA semi-IPN hydrogel underwent, reversibly, remarkable changes in swelling degree in response to the variation of pH, In the low-pH range, the hydrogel showed a lower swelling degree; with an increment in pH, the hydrogel was highly swollen, which is considered to originate from the complexation and de-complexation between gelatin and DNA, as was verified by turbidity measurements. Higher contents of DNA result in an increase in the swelling degree, which is presumably due to the easy outward expansion of free DNA moieties. The permeability coefficient, P, for a model molecule, cimetidine, through the semi-IPN hydrogel membranes was determined in pH 1.0 and pH 12.0 buffer solutions. The results show that the permeation of cimetidine is responsive to pH change, and an evident variation in the P values occurs in response to the pH of the media.

Original languageEnglish (US)
Pages (from-to)675-680
Number of pages6
JournalPolymer International
Volume53
Issue number6
DOIs
StatePublished - Jun 1 2004

Fingerprint

Interpenetrating polymer networks
Hydrogel
Gelatin
Hydrogels
DNA
Swelling
Cimetidine
Complexation
Hydraulic conductivity
Glutaral
Turbidity
Permeation
Infrared spectroscopy
Buffers
Membranes
Molecules

Keywords

  • DNA
  • Gelatin
  • Hydrogel
  • Semi-interpenetrating polymer network

ASJC Scopus subject areas

  • Polymers and Plastics

Cite this

A novel pH-sensitive gelatin-DNA semi-interpenetrating polymer network hydrogel. / Liu, Wen Guang; Li, Xiaowei; Ye, Gui Xiang; Sun, Shu Jun; Zhu, Dunwan; Yao, Kang De.

In: Polymer International, Vol. 53, No. 6, 01.06.2004, p. 675-680.

Research output: Contribution to journalArticle

Liu, Wen Guang ; Li, Xiaowei ; Ye, Gui Xiang ; Sun, Shu Jun ; Zhu, Dunwan ; Yao, Kang De. / A novel pH-sensitive gelatin-DNA semi-interpenetrating polymer network hydrogel. In: Polymer International. 2004 ; Vol. 53, No. 6. pp. 675-680.
@article{74db6739bae64a069e13e98986136ad4,
title = "A novel pH-sensitive gelatin-DNA semi-interpenetrating polymer network hydrogel",
abstract = "Gelatin and DNA were mixed together in various ratios followed by the addition of glutaraldehyde as a cross-linker. FT-IR spectroscopy confirmed the formation of a semi-interpenetrating polymer network (semi-IPN) between the gelatin and DNA. The gelatin-DNA semi-IPN hydrogel underwent, reversibly, remarkable changes in swelling degree in response to the variation of pH, In the low-pH range, the hydrogel showed a lower swelling degree; with an increment in pH, the hydrogel was highly swollen, which is considered to originate from the complexation and de-complexation between gelatin and DNA, as was verified by turbidity measurements. Higher contents of DNA result in an increase in the swelling degree, which is presumably due to the easy outward expansion of free DNA moieties. The permeability coefficient, P, for a model molecule, cimetidine, through the semi-IPN hydrogel membranes was determined in pH 1.0 and pH 12.0 buffer solutions. The results show that the permeation of cimetidine is responsive to pH change, and an evident variation in the P values occurs in response to the pH of the media.",
keywords = "DNA, Gelatin, Hydrogel, Semi-interpenetrating polymer network",
author = "Liu, {Wen Guang} and Xiaowei Li and Ye, {Gui Xiang} and Sun, {Shu Jun} and Dunwan Zhu and Yao, {Kang De}",
year = "2004",
month = "6",
day = "1",
doi = "10.1002/pi.1358",
language = "English (US)",
volume = "53",
pages = "675--680",
journal = "Polymer International",
issn = "0959-8103",
publisher = "John Wiley and Sons Ltd",
number = "6",

}

TY - JOUR

T1 - A novel pH-sensitive gelatin-DNA semi-interpenetrating polymer network hydrogel

AU - Liu, Wen Guang

AU - Li, Xiaowei

AU - Ye, Gui Xiang

AU - Sun, Shu Jun

AU - Zhu, Dunwan

AU - Yao, Kang De

PY - 2004/6/1

Y1 - 2004/6/1

N2 - Gelatin and DNA were mixed together in various ratios followed by the addition of glutaraldehyde as a cross-linker. FT-IR spectroscopy confirmed the formation of a semi-interpenetrating polymer network (semi-IPN) between the gelatin and DNA. The gelatin-DNA semi-IPN hydrogel underwent, reversibly, remarkable changes in swelling degree in response to the variation of pH, In the low-pH range, the hydrogel showed a lower swelling degree; with an increment in pH, the hydrogel was highly swollen, which is considered to originate from the complexation and de-complexation between gelatin and DNA, as was verified by turbidity measurements. Higher contents of DNA result in an increase in the swelling degree, which is presumably due to the easy outward expansion of free DNA moieties. The permeability coefficient, P, for a model molecule, cimetidine, through the semi-IPN hydrogel membranes was determined in pH 1.0 and pH 12.0 buffer solutions. The results show that the permeation of cimetidine is responsive to pH change, and an evident variation in the P values occurs in response to the pH of the media.

AB - Gelatin and DNA were mixed together in various ratios followed by the addition of glutaraldehyde as a cross-linker. FT-IR spectroscopy confirmed the formation of a semi-interpenetrating polymer network (semi-IPN) between the gelatin and DNA. The gelatin-DNA semi-IPN hydrogel underwent, reversibly, remarkable changes in swelling degree in response to the variation of pH, In the low-pH range, the hydrogel showed a lower swelling degree; with an increment in pH, the hydrogel was highly swollen, which is considered to originate from the complexation and de-complexation between gelatin and DNA, as was verified by turbidity measurements. Higher contents of DNA result in an increase in the swelling degree, which is presumably due to the easy outward expansion of free DNA moieties. The permeability coefficient, P, for a model molecule, cimetidine, through the semi-IPN hydrogel membranes was determined in pH 1.0 and pH 12.0 buffer solutions. The results show that the permeation of cimetidine is responsive to pH change, and an evident variation in the P values occurs in response to the pH of the media.

KW - DNA

KW - Gelatin

KW - Hydrogel

KW - Semi-interpenetrating polymer network

UR - http://www.scopus.com/inward/record.url?scp=2542436779&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2542436779&partnerID=8YFLogxK

U2 - 10.1002/pi.1358

DO - 10.1002/pi.1358

M3 - Article

VL - 53

SP - 675

EP - 680

JO - Polymer International

JF - Polymer International

SN - 0959-8103

IS - 6

ER -