A novel adjuvant for vaccine development in the aged

Edward L. Morgan, Marilyn L. Thoman, Sam D. Sanderson, Joy A. Phillips

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

A conformationally-biased, response-selective agonist of human C5a65-74 (EP67) activated antigen presenting cells (APC) from aged C57Bl/6 mice in vitro and the generation of antigen (Ag)-specific antibody (Ab) responses in aged mice in vivo. EP67, induced the release of the pro-inflammatory cytokines IL-6, TNFα, and INFγ from splenic APCs obtained from both aged and young mice. Both aged and young mice produced high Ag-specific IgG Ab titers when immunized with EP67-containing vaccines to ovalbumin (OVA-EP67) and to a protein (rPrp1) from the cell wall of Coccidioides (rPrp1-EP67). Immunization with EP67-containing vaccines resulted in higher IgG titers in both young and aged mice compared to mice immunized with OVA adsorbed to alum (OVA/alum) and Prp1 admixed with CpG (rPrp1 +CpG). Aged and young mice immunized with the EP67-containing vaccines generated higher titers of IgG1 and IgG2b relative to their aged-matched counterparts immunized with OVA/alum or Prp1 +CpG. These results indicate that EP67 induces humoral immunity in aged mice not obtainable with alum and CpG. These results support the use of EP67 as a potential vaccine adjuvant suited to the elderly.

Original languageEnglish (US)
Pages (from-to)8275-8279
Number of pages5
JournalVaccine
Volume28
Issue number52
DOIs
StatePublished - Dec 6 2010

Fingerprint

vaccine development
adjuvants
Vaccines
mice
alum
Immunoglobulin G
vaccines
Coccidioides
antigens
Antigens
vaccine adjuvants
antibodies
antigen-presenting cells
ovalbumin
Ovalbumin
Antigen-Presenting Cells
Humoral Immunity
humoral immunity
interleukin-6
Cell Wall

Keywords

  • Adjuvant
  • Aging
  • Antibody response responses
  • Cytokines
  • Immunization
  • Vaccine

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

Cite this

Morgan, E. L., Thoman, M. L., Sanderson, S. D., & Phillips, J. A. (2010). A novel adjuvant for vaccine development in the aged. Vaccine, 28(52), 8275-8279. https://doi.org/10.1016/j.vaccine.2010.10.008

A novel adjuvant for vaccine development in the aged. / Morgan, Edward L.; Thoman, Marilyn L.; Sanderson, Sam D.; Phillips, Joy A.

In: Vaccine, Vol. 28, No. 52, 06.12.2010, p. 8275-8279.

Research output: Contribution to journalArticle

Morgan, EL, Thoman, ML, Sanderson, SD & Phillips, JA 2010, 'A novel adjuvant for vaccine development in the aged', Vaccine, vol. 28, no. 52, pp. 8275-8279. https://doi.org/10.1016/j.vaccine.2010.10.008
Morgan EL, Thoman ML, Sanderson SD, Phillips JA. A novel adjuvant for vaccine development in the aged. Vaccine. 2010 Dec 6;28(52):8275-8279. https://doi.org/10.1016/j.vaccine.2010.10.008
Morgan, Edward L. ; Thoman, Marilyn L. ; Sanderson, Sam D. ; Phillips, Joy A. / A novel adjuvant for vaccine development in the aged. In: Vaccine. 2010 ; Vol. 28, No. 52. pp. 8275-8279.
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