A nodule-specific gene family from Alnus glutinosa encodes glycine- and histidine-rich proteins expressed in the early stages of actinorhizal nodule development

Katharina Pawlowski, Paul Twigg, Svetlana Dobritsa, Changhui Guan, Beth C. Mullin

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Two cDNAs representing different members (agNt84 and ag164) of a gene family encoding glycine- and histidinerich proteins have been isolated from cDNA libraries from Alnus glutinosa root nodules. Expression of the corresponding genes could only be detected in nodules. With in situ hybridization, the expression in nodules was found to occur in young, infected cells of the prefixation zone (zone 2). The encoded proteins contain putative signal peptides for targeting to the endomembrane system, sharing sequence similarity with signal peptides from plant glycinerich proteins, among them nodulin 24, a nodule-specific protein from soybean. This similarity suggests that, analogous to nodulin-24, proteins encoded by agNt84/ag164 may be located at the interface between the host plant membrane and the matrix surrounding the endosymbiont. The 3' untranslated regions of the cDNAs contain unusual poly(AT)(n) stretches that may play a role in the regulation of RNA stability. The protein encoded by agNt84 cDNA was expressed in Escherichia coil as a fusion with maltose-binding protein, and was shown to have the ability to bind to a nickel-chelating resin, indicating that it may function as a metal-binding protein.

Original languageEnglish (US)
Pages (from-to)656-664
Number of pages9
JournalMolecular Plant-Microbe Interactions
Volume10
Issue number5
DOIs
StatePublished - Jul 1997

Fingerprint

Alnus
Alnus glutinosa
histidine
glycine (amino acid)
Glycine
Genes
Complementary DNA
nodulins
Protein Sorting Signals
signal peptide
Proteins
genes
proteins
binding proteins
Maltose-Binding Proteins
Escherichia
Plant Proteins
Soybean Proteins
endomembrane system
RNA Stability

Keywords

  • Actinorhizal
  • Nitrogen fixation

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science

Cite this

A nodule-specific gene family from Alnus glutinosa encodes glycine- and histidine-rich proteins expressed in the early stages of actinorhizal nodule development. / Pawlowski, Katharina; Twigg, Paul; Dobritsa, Svetlana; Guan, Changhui; Mullin, Beth C.

In: Molecular Plant-Microbe Interactions, Vol. 10, No. 5, 07.1997, p. 656-664.

Research output: Contribution to journalArticle

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